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AA Met & Urea
USCSOM: Amino Acid Metabolism and Urea Cycle
Question | Answer |
---|---|
Dietary proteins are the primary source of ______ metabolized by the body. | nitrogen |
What catalyzes the activation of pepsin? | H+ |
What catalyzes the activation of trypin? | enteropeptidase |
What catalyzes the activation of chymotrypsin? | trypsin |
What catalyzes the activation of elastase? | trypsin |
What catalyzes the activation of carboxypeptidases? | trypsin |
What is the general name for digestive proteases that hydrolyze peptide bonds within the chains? | endopeptidases |
What are some examples of endopeptidases? | pepsin, trypsin, chymotrypsin, elastase |
What are the two exopeptidases? | aminopeptidase and carboxypeptidases |
What amino acids do pepsin cleave? | aromatic and acidic AAs |
What AA do trypsin cleave? | Basic: Arg, Lys |
What AA do chymotrypsin cleave? | Hydrophobic: Phe, Tyr, Trp, Leu |
What AA do elastase cleave? | Small AAs: Ala, Gly, Ser |
Describe trans-cellular transport mediated by secondary active transport | Na-dependent AA transporters; Na-K ATPase pump; AA facilitated diffusion |
In a fasting state, AA are converted mainly into what 2 AAs? | Ala, Gln |
What AA plays a central role in both AA synthesis and catabolism? | Glu (Glutamate) |
During AA synthesis, Glu donates/takes Amino N via transamination? | donates |
During AA catabolism, Glu donates/takes Amino N via transamination? | takes |
What to transamination enzymes play a role in Glutamate-AA synthesis and catabolism? | GDH - glutamate dehydrogenase |
PLP is derived from what nutrient? | vitamin B6 |
The glutamate dehydrogenase reaction uses what cofactor? | NAD or NADP |
Excess amino acid N is converted to what toxic substance? | ammonia |
Ammonia is converted to what less toxic substance at physiological pH? | ammonium |
What organ converts ammonia to urea? | liver |
What is the major nitrogenous urinary excretory product? | urea |
What 2 AAs require PLP as a cofactor to form NH4+? | Serine & Threonine |
What AAs directly contribute their N to the formation of NH4+? | Glutamate, Glutamine, Asparagine, Serine, Threonine, Histidine |
What AA plays a major transport role for transport of ammonia in a non-toxic form for Urea Synthesis? | alpha-kG to Glu to Gln |
What 2 AAs do nitrogen come from the form urea? | Glu and Asp |
What is CPS1? | carbamoyl phophate sythetase 1 |
What are the products in the urea cycle? | ammonium, carbamoyl phosphate, citrulline, argininosuccinate, arginine, ornithine |
What enzyme from the urea cycle is unique to the liver? | arginase |
What is NAG? | N-acetyl glutamate |
What is the role of NAG? | formed from a build-up of ARg |
What is the effect of fasting on urea excretion? | increases during fasting |
What is the ratio of Ala:glucose:urea | 2:1:1 |