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SIB2004 6

Enzyme Classification & Nomenclature

QuestionAnswer
How are enzymes named? Enzymes are often known by commmon names obtained by adding the suffix "ase" to the name of the name of substrate or to the reaction they catalyse.
What does Amylase catalyse? Catalyses the hydrolysis of α-amylose.
What does Urease catalyse? Catalyses the hydrolysis of urea.
What does Xanthine oxidase catalyse? Catalyses the oxidation of xanthine.
What does Glucose-6-phosphate catalyse? Catalyses the hydrolysis of phosphate from glucose.
Who devised a naming and numbering system? The Enzyme Commission (EC) of the International Union of Biochemistry and Molecular Biology (IUBMB).
What does IUBMB stand for? International Union of Biochemistry and Molecular Biology.
What does EC stand for? Enzyme Commission.
How many major classes can enzymes be divided into? 6.
What does sub-groups and sub-subgroups do? Define their functions more precisely.
What are the classes of enzymes? Hydrolases Isomerases Ligases Lyases Oxidoreductases Transferases
What do oxidoreductases catalyse? Catalyse oxidation-reduction reactions.
What are examples of oxidoreductases? Dehydrogenases Oxidases Peroxidases
What is the formula for oxidoreducatase? A– + B → A + B- A+ + B → A + B+ AH + B → A + BH
What do transferases catalyse? Catalyse transfer of functional group from one molecule to another.
What are examples of transferases? Transaminases Transmethylases
What is the formula for transferase? A-X + B → A + X-C
What do hydrolases catalyse? Catalyse hydrolytic cleavage (hydrolysis reactions).
What are examples of hydrolases? Digestive enzymes such as: Sucrase Amylase Maltase Lactase
What is the formula for hydrolase? A–B + H2O → A–OH + B–H
What do lyases catalyse? Catalyse the removal of a group or addition of a group to a double bond, or other cleavages involving electron rearrangement.
What is an example of a lyase? Decarboxylase
What is the formula for lyase? XY=A-B → A=B + X-Y
What do isomerases catalyse? Catalyses intramolecular rearrangement.
What are examples of isomerases? Racemases Cis-trans isomerases
What is the formula for isomerases? XY=A-B → YX=A-B
What do ligases catalyse? Catalyse reactions in which two molecules are joined.
What is an example of a ligase? DNA ligase
What is the formula for ligases? A + B → A-B
How many parts (numbers) has the EC of the IUBMB given to each enzyme? 4
What do the first three numbers of an enzyme indicate? Major Class Sub Class Sub-sub Class
What is the last number of an enzyme and what does it indicate? A serial number, indicating the order in which each enzume is added to the list, which is continually growing.
Example of EC Numbers on Triose phosphate isomerase is listed as EC 5.3.1.1. It's an isomerase d and in the third 3 subclass. It is in the first 1 sub-subclass (those that interconvert aldoses and ketoses) and is the first 1 entry (of 19 so far) in this sub-subclass.
Created by: Groberts00 on 2013-02-23



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