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BBBFMPT
Question | Answer |
---|---|
Bordetella | obligate aerobe extracellular pathogen oxidase positive (+) asaccharolytic transmission is inhalation |
Bordetella bronchiseptica | can culture on blood/MacConkey agar causes bronchopneumonia in dogs, cats, and pigs causes atophic rhinitis in pigs causes tracheobronchitis in dogs "kennel cough" |
Bordetella pertussis | fastidious human-adapted causes tracheobronchitis "whooping cough" must culture on BORDET-GENOU agar |
Brucella species | B. abortus-->cows, dogs, horses, humans B. canis-->dogs, humans B. melitensis-->sheep, humans B. ovis-->sheep ONLY B. suis-->pigs, humans ______________________ CO2 requirements H2S production urease activity inhibited by fuschin and thionin |
Brucella smooth species | HAVE somatic antigens B. abortus B. melitensis B. suis |
Brucella rough species | B. canis B. ovis |
Brucella serologic tests for smooth species | CARD TEST: tests for IgG, acid pH inactivates IgM 2-MERCAPTOETHANOL: tests for IgG, 2-mercaptoethanol inactivates IgM RIVANOL TEST: tests for IgG, rivanol inactivates IgM |
Brucella infection basics | INTRACELLULAR pathogens can penetrate INTACT mm's primary mode of transmission = ingestion |
Brucella abortus | bovine-adapted fastidious, ox +, saccharolytic abortion in 5th month of gestation orchitis in males |
Brucella canis | DOG ox +, saccharolytic abortion in 2nd and 3rd month orchitis/epididymitis |
Brucella melitensis | SHEEP ox +, saccharolytic abortion orchitis/epididymitis |
Brucella ovis | SHEEP more of an STD? ox -, asaccharolytic subclinical in females (some abortions) epididymitis, orchitis, poor sperm quality in males |
Brucella suis | PIG abortion, stillborn, weak piglets usually subclinical in males |
Bovine Brucellosis | bacteria do not proliferate before female reaches sexual maturity phagocytized by neutrophils/macrophages, transported to tissues by macrophages growth enhanced by erythritol (produced in prego uterus) only cell-mediated immunity helps |
Bovine Brucellosis DX serology tests | standard tube agglutination, card test, rivanol, 2-mercaptoethanol, compliment-fixation, milk ring test (use B. abortus antigen stained w/ tetrazolium dye on pooled milk sample, antibodies will agglutinate the antigens & rise to the surface --> blue ring) |
Burkholderia | blood agar ox + saccharolytic obligate aerobe includes Burkholderia mallei and Burkholderia pseudomallei |
Burkholderia mallei | HORSE nonmotile causes cutaneous, pulmonary, and ulcerative glanders (nodules w/ yellow caseous purulent material) use mallein to ID infected horses (+ rxn is swelling at site in injection) |
Burkholderia pseudomallei | wide host range motile with POLAR flagella causes meliodosis (similar to glanders) |
Francisella (tularensis) | can penetrate INTACT mm's wide host range fastidious -- cysteine ox - saccharolytic obligate aerobe causes tularemia transmission mostly direct contact distinguish subspp. on ability to metabolize glycerol |
Moraxella (bovis) | blood agar ox + asaccharolytic obligate aerobe high solar radiation BOVINE keratoconjunctivitis (direct contact) only ROUGH strains WITHOUT somatic antigens are virulent |
Pseudomonas (aeruginosa) | motile, POLAR blood/MacConkey agar obligate aerobe* terminal e- acceptor is NITROGEN purulent green exudate life-threatening in burn pt's |
Taylorella (equigenitalis) | fastidious (X&V, capnophilic) ox + saccharolytic FACULTATIVE anaerobe EQUINE CONTAGIOUS METRITIS: female repro. tract, STD, infertility earlier on in process, use serologic test 2-6 wks PI to DX |