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Enterobacteriaceaee
| Question | Answer |
|---|---|
| Shared characteristics | G- bacilli can culture on blood or MacConkey agar facultative anaerobes saccharolytic oxidase negative (-) |
| Genera included | Citrobacter Enterobacter Escherichia Klebsiella Proteus Salmonella Shigella Yersinia |
| Citrobacter and Enterobacter | motile human/animal EXTRAINTESTINAL pathogen opportunistic mixed cultures |
| Escherichia coli serovars | based on protein-composed fimbral (F) antigens CFA-I through CFA-IV cause human disease F-4, -5, -6, and -41 cause porcine disease |
| Enterohemorrhagic E. coli | motile lower small intestine bloody diarrhea Shigalike toxinf I and II most common serovar = O157:H7 |
| Enteroinvasive E. coli | similar to Shigella dysentery large intestines and colon |
| Enteropathogenic E. coli | entercolitis similar to Salmonella enterotoxins and shigalike toxins |
| Human Enterotoxigenic E. coli | transmission: ingestion or overproliferation of normal flora watery diarrhea and dehydration --> death stomach and small intestine distended with gas and fluid most produce complete hemolysis |
| Human Enterotoxigenic E. coli virulence factors | FIMBRAE: coded for on plasmids, allow colonization HEAT-LAIBLE: destroyed @ 56C for 30m. Holotoxin. MW 85,000. 1 A increases cAMP inhibits Na+ channel. The 5B subunits bind HEAT-STABLE A&B: nonantigenic, MW=2,000. A increases cGMP. B is anti-absorptive |
| Enterotoxigenic E. coli w/ heat-laible enterotoxins DX | LIGATED ILEAL LOOP OF LIVE RABBITS: inject w/ supernatant, see fluid accumulation. *can also use for heat-stable* TISSUE CULTURE METHOD W/ MOUSE ADRENAL OR HAMSTER OVARY: expose tissue to supernatant, look for morpholigical response to incr. of cAMP |
| Human Enterotoxigenic E. coli w/ heat-laible enterotoxins DX | INFANT MOUSE TEST inject supernatant into infant mouse stomach, wait 4 hr, look for fluid accumulation :( |
| Klebsiella | nonmotile opportunistic EXTRAINTESTINAL infections (cystitis, mastitis, metritis) pure culture potent endotoxin --> endotoxic shock |
| Proteus | motile opportunistic EXTRAINTESTINAL infections mixed cultures cause UTI's in humans/domestic animals |
| Salmonella (basic info) | all but avian-adapted are motile w/ PERITRICHOUS flagella nonhemolytic on blood agar colorless on MacConkey |
| Salmonella Serovars | based on O antigens (#), H phase 1 antigens (a, b, c...) and H pahse 2 antigens (#) S. Dublin and S. Typhi also have VI carbohydrate capsular antigens NOT used for serotyping |
| Salmonella Cholerasuis | pigs acute septicemia with endotoxemia, fever high MR or chronic pneumonia enteritis is rare |
| Salmonella Dublin | cow acute enteritis with bloody diarrhea esp. feedlot cattle that have been shipped |
| Salmonella Typhi | HUMAN TYPHOID transmission: ingestion septicemia, fever, GI symptoms VI antigen important unlike other salmonella serovars, ANAREOGENIC in glucose broth |
| Salmonalla Typhimurium | in cows: similar to S. Dublin in horses: entercolitis, can localize to other tissues, high MR, antibiotic-induced in pigs: causes Porcine Salmonellae Entercolitis |
| S. Typhimurium and Porcine Entercolitis | young pigs, four stages: 1)ingestion 2)infection/invasion of GI tract 3)proliferation in intestinal wall 4)bacteremia/septicemia ileum, cecum, coloncytotoxins, enterotoxins, LPS Serogroup B, C, D, or E; use agglutination tests with sero-specific antiser |
| Shigella dysenteriae | nonmotile no hemolysis colorledd MacConkey colonies causes Human Bacilliary Dysentery |
| Human Bacilliary Dyesntery | ingestion watery feces w/ blood + mucus polysaccharide capsule, LPS, cytotoxins (holotoxins, MW 62,000, inhibit protein synth in euk cells, also called "verotoxins", include shiga tox I + II), enterotoxins cecum + lg. intestine |
| Yersinia entercolitica | causes enteritis in humans (milk/meat ingestion) swine reservoir ileitis, mesenteric lymphadenitis, septicemia diarrhea temp-dependent motility, peritrichous falgella |
| Yersinia pseudotuberculosis | psuedoTB in some animals pseudoTB in humans: lesions w/ caseous necrosis temp-dependent motility w/ peritrichous flagella |
| Yersinia pestis | nonmotile extraintestinal pathogen of rodents causes human plague capsule |
Created by:
jesters
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