IMViC tests Word Scramble

Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

Normal Size Small Size show me how

Question	Answer
The IMViC tests are for gram negative bacteria called	enterobacteriaceae
gram negative bacteria are shaped like	single rod bacilli
After smearing enterobacteriaceae on a slide, 5 drops of Kovac's Reagent test	Indole
What does the Indole test for?	the enzyme tryptophanase
What are the products of tryptophanase in the indole test?	indole pyruvate ammonia
positive indole test colors	deep red
negative indole test colors	yellow
all bacteria in the enterobacteriaceae family	ferment glucose
If the MR test is red, what does that mean?	positive for fermentaion that produced mixed acids
If the VP test is positive red, what does that mean?	positive for fermentation that produced acetoin.
What does the VP stand for in the acetoin test?	Vogues-Proskauer
The VP test and the MR test both cannot have same	result
All bacteria in the enterobacteriaceae family	ferment glucose
the ablity of enterobacteriaceae to utilize citrate as their sole carbon source	citrate test
In the citrate test, what would the ph environment be like if citrate were the only carbon source?	Alkaline with a ph above 7.5
a negative citrate result in colored	green
a positive citrate result is colored	blue
green with luxurious growth can also be a positive	citrate test
Why do we use an innoculating needle for the TSI slant?	aerobic and anaerobic growth
In a TSI slant, the top growth is	aerobic and acidic
In a TSI slant, the bottom gowth is	anaerobic and alkaline
In the TSI slant bubbles at the bottom of the slant indicate	fermentation gas
The purpose of the TSI slant is to show	acid growth alkaline growth fermentation bubbles
A positive aerobic TSI test shows	red on the top
a positive anaerobic test will be colored	yellow on the butt
a positive anaerobic test may include fermentation	bubbles and be yellow
What does the urea test for?	exoenzyme urase
What do the MR and VP tests look for?	Selective for gram negative Differential for acid and acetoin
What does the indole test look for?	tryptophan>trytophanase>indole>ammonia>pyruvate
The MCConkey plate tests for lactose fermentation, and if it is present, the colonies will be colored	pink
XLD test is for sugar fermentaion, and if it is present it will be colored	yellow for acid production
Why are microflora helpful?	strong immune system
How do I find out if I have a gram positive or a gram negaive bacteria?	catalase test
Bacteria that are always catalase positive are called	Staphylococcus
What does catalase enzyme do?	it breaks down hydrogen peroxide to water and oxygen.
one slide, one drop of water, one drop of rabbit plasma	how to set up the coagulase enzyme test
The result of the coagulase test should be	clumping if positive
The bacteria that is positive for the coagulase test is caled	S. aureus
The coagulase enzyme cause	fibrinogen
is soluable in the blood	fibrinogen
is insoluable in the blood	fibrin
causes tiny blood clots during an infection to help hide from the body's white cells	fibrin
What does the fibrin coagulate?	blood plasma
phenol red is a	ph indicator
What specific vitamins do intestinal microflora produce tha is helpful t the human body?	vitamin B vitamin K
Why do bacteria produce the enzyme coagulase	to hide inside the clot as a protective barrier durig an infection

Created by: 1544741150