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IMViC tests
microbiology
| Question | Answer |
|---|---|
| The IMViC tests are for gram negative bacteria called | enterobacteriaceae |
| gram negative bacteria are shaped like | single rod bacilli |
| After smearing enterobacteriaceae on a slide, 5 drops of Kovac's Reagent test | Indole |
| What does the Indole test for? | the enzyme tryptophanase |
| What are the products of tryptophanase in the indole test? | indole pyruvate ammonia |
| positive indole test colors | deep red |
| negative indole test colors | yellow |
| all bacteria in the enterobacteriaceae family | ferment glucose |
| If the MR test is red, what does that mean? | positive for fermentaion that produced mixed acids |
| If the VP test is positive red, what does that mean? | positive for fermentation that produced acetoin. |
| What does the VP stand for in the acetoin test? | Vogues-Proskauer |
| The VP test and the MR test both cannot have same | result |
| All bacteria in the enterobacteriaceae family | ferment glucose |
| the ablity of enterobacteriaceae to utilize citrate as their sole carbon source | citrate test |
| In the citrate test, what would the ph environment be like if citrate were the only carbon source? | Alkaline with a ph above 7.5 |
| a negative citrate result in colored | green |
| a positive citrate result is colored | blue |
| green with luxurious growth can also be a positive | citrate test |
| Why do we use an innoculating needle for the TSI slant? | aerobic and anaerobic growth |
| In a TSI slant, the top growth is | aerobic and acidic |
| In a TSI slant, the bottom gowth is | anaerobic and alkaline |
| In the TSI slant bubbles at the bottom of the slant indicate | fermentation gas |
| The purpose of the TSI slant is to show | acid growth alkaline growth fermentation bubbles |
| A positive aerobic TSI test shows | red on the top |
| a positive anaerobic test will be colored | yellow on the butt |
| a positive anaerobic test may include fermentation | bubbles and be yellow |
| What does the urea test for? | exoenzyme urase |
| What do the MR and VP tests look for? | Selective for gram negative Differential for acid and acetoin |
| What does the indole test look for? | tryptophan>trytophanase>indole>ammonia>pyruvate |
| The MCConkey plate tests for lactose fermentation, and if it is present, the colonies will be colored | pink |
| XLD test is for sugar fermentaion, and if it is present it will be colored | yellow for acid production |
| Why are microflora helpful? | strong immune system |
| How do I find out if I have a gram positive or a gram negaive bacteria? | catalase test |
| Bacteria that are always catalase positive are called | Staphylococcus |
| What does catalase enzyme do? | it breaks down hydrogen peroxide to water and oxygen. |
| one slide, one drop of water, one drop of rabbit plasma | how to set up the coagulase enzyme test |
| The result of the coagulase test should be | clumping if positive |
| The bacteria that is positive for the coagulase test is caled | S. aureus |
| The coagulase enzyme cause | fibrinogen |
| is soluable in the blood | fibrinogen |
| is insoluable in the blood | fibrin |
| causes tiny blood clots during an infection to help hide from the body's white cells | fibrin |
| What does the fibrin coagulate? | blood plasma |
| phenol red is a | ph indicator |
| What specific vitamins do intestinal microflora produce tha is helpful t the human body? | vitamin B vitamin K |
| Why do bacteria produce the enzyme coagulase | to hide inside the clot as a protective barrier durig an infection |
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