Lecture 3 Word Scramble
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| Question | Answer |
| What is MNase digestion and mapping? | positioning of nucleosomes relative to known sequence; small |
| What is DNase digestion and maping? | access to the DNA on or near nucleosomes, distortions; bigger |
| What is Electrophoretic Mobility Shift Assay (EMSA)? | DNA bound to protein will run more slowly in a non-denaturing PAGE gel |
| What is the SWI/SNF class of remodelers? | large complexes containing actin proteins; cause sliding and ejection of histones; binds acetylated histones |
| What is the SWI/Snf function? | required for gene expression; 80 bp at a time |
| What is the RSC function? | required for kinetochore function and cohesion in the arms of the kinetochore. |
| What is the ISWI class of remodelers? | large protein complex that makes small changes in nucleosome assembly and spacing; 10 bp at a time; binds unmodified histones with SANT |
| What is the CHD class of remodelers? | provides a link between histone modification and covalent modification; binds methylated histones |
| What is the split ATPase class of remodelers? | Ino80 and SWR1 |
| What is the function of Ino80? | repairs damaged DNA by phosphorylated histones |
| What is the function of SWR1? | swaps variant histones into the nucleosomes of actively transcribed genes; H2AZ for H2A |
| Where does acetylation occur? | lysine |
| Where does methylation occur? | lysine and arginine |
| Where does phosphorylation occur? | serine and threonine |
| Where does ubiquitylation occur? | lysine |
| What is ubiquitin? | signaling module; multiple targets protein for degradation; single functions as a switch |
| What is Western blot? | SDS-PAGE protein separation; antibody detected by a chemically labeled secondary antibody |
| What is Immunoprecipitation (IP)? | extract treated with antibody; antibody is isolated and bound to target protein |
| What is Chromatin Immunoprecipitation (ChIP)? | isolates proteins with DNA associated |
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