microbiology
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| The IMViC tests are for gram negative bacteria called | enterobacteriaceae
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| gram negative bacteria are shaped like | single rod bacilli
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| After smearing enterobacteriaceae on a slide, 5 drops of Kovac's Reagent test | Indole
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| What does the Indole test for? | the enzyme tryptophanase
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| What are the products of tryptophanase in the indole test? | indole
pyruvate
ammonia
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| positive indole test colors | deep red
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| negative indole test colors | yellow
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| all bacteria in the enterobacteriaceae family | ferment glucose
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| If the MR test is red, what does that mean? | positive for fermentaion that produced mixed acids
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| If the VP test is positive red, what does that mean? | positive for fermentation that produced acetoin.
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| What does the VP stand for in the acetoin test? | Vogues-Proskauer
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| The VP test and the MR test both cannot have same | result
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| All bacteria in the enterobacteriaceae family | ferment glucose
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| the ablity of enterobacteriaceae to utilize citrate as their sole carbon source | citrate test
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| In the citrate test, what would the ph environment be like if citrate were the only carbon source? | Alkaline with a ph above 7.5
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| a negative citrate result in colored | green
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| a positive citrate result is colored | blue
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| green with luxurious growth can also be a positive | citrate test
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| Why do we use an innoculating needle for the TSI slant? | aerobic and anaerobic growth
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| In a TSI slant, the top growth is | aerobic and acidic
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| In a TSI slant, the bottom gowth is | anaerobic and alkaline
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| In the TSI slant bubbles at the bottom of the slant indicate | fermentation gas
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| The purpose of the TSI slant is to show | acid growth
alkaline growth
fermentation bubbles
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| A positive aerobic TSI test shows | red on the top
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| a positive anaerobic test will be colored | yellow on the butt
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| a positive anaerobic test may include fermentation | bubbles and be yellow
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| What does the urea test for? | exoenzyme urase
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| What do the MR and VP tests look for? | Selective for gram negative
Differential for acid and acetoin
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| What does the indole test look for? | tryptophan>trytophanase>indole>ammonia>pyruvate
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| The MCConkey plate tests for lactose fermentation, and if it is present, the colonies will be colored | pink
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| XLD test is for sugar fermentaion, and if it is present it will be colored | yellow for acid production
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| Why are microflora helpful? | strong immune system
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| How do I find out if I have a gram positive or a gram negaive bacteria? | catalase test
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| Bacteria that are always catalase positive are called | Staphylococcus
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| What does catalase enzyme do? | it breaks down hydrogen peroxide to water and oxygen.
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| one slide, one drop of water, one drop of rabbit plasma | how to set up the coagulase enzyme test
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| The result of the coagulase test should be | clumping if positive
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| The bacteria that is positive for the coagulase test is caled | S. aureus
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| The coagulase enzyme cause | fibrinogen
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| is soluable in the blood | fibrinogen
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| is insoluable in the blood | fibrin
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| causes tiny blood clots during an infection to help hide from the body's white cells | fibrin
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| What does the fibrin coagulate? | blood plasma
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| phenol red is a | ph indicator
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| What specific vitamins do intestinal microflora produce tha is helpful t the human body? | vitamin B
vitamin K
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| Why do bacteria produce the enzyme coagulase | to hide inside the clot as a protective barrier durig an infection
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