-.-17
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| 1. The most recent techniques developed in the biological sciences allow the manipulation of DNA with the ultimate goal of intervening directly with the _______ fate of organisms. | show 🗑
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| show | plasmids
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| 3. Enzymes that cleave DNA at specific sites are called ______________. | show 🗑
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| show | cloning
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| 5. Single-stranded complementary tails that are produced by restriction digestion are called ______ ends. | show 🗑
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| show | vectors
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| show | electrophoresis
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| 8. The _________ from Agrobacterium tumefaciens is used in genetic engineering involving crop plant genes. | show 🗑
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| 9. _________ is a human protein synthesized in small amounts that can dissolve blood clots. | show 🗑
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| 10. A ______ vaccine is produced by using only a part of the viral genome. | show 🗑
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| show | viruses
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| show | plasmids.
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| show | restriction enzyme.
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| 14. Single-stranded ends generated by the same restriction enzyme are complementary to each other. The can be joined together, | show 🗑
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| show | ligase enzyme.
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| show | hybridization.
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| show | using any cell as a vector.
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| show |
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| independently of the chromosome, and | show 🗑
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| 19. DNA fragments complementary to the DNA being investigated are referred to as | show 🗑
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| 20. Restriction enzymes are | show 🗑
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| show | reverse transcriptase.
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| 22. Restriction enzymes recognize DNA sequences that are | show 🗑
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| 23. The ends of DNA fragments produced by the same restriction enzyme can be joined together by another enzyme, | show 🗑
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| show | plasmid.
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| show | integration of the entire bacterial chromosome
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| 26. The most common way to follow bacterial transformation with a plasmid is by | show 🗑
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| show | addition of new Taq polymerase after each cycle.
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| show | specific complimentary single stranded DNA.
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| 29. When electrical current is applied during a gel electrophoresis procedure, the DNA fragments are separated by | show 🗑
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| show | beta-galactosidase
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| 31. A commercially significant human protein now produced in bacteria is | show 🗑
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| show | denaturation, annealing of primers, and then primer extension.
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| 33. A plasmid used to carry genes into crop plants is | show 🗑
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| show | Bacillus thuringiensis.
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| 35. Scientists can distinguish between DNA of different individuals, thus making this information useful in criminal investigations. The technique used is called | show 🗑
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| show | incorporate part of the protein—polysaccharide coat of either the herpes or hepatitis B with the cowpox virus.
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| show | to clone the perfect human being.
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| 38. Agrobacterium is more difficult to use in genetic engineering procedures conducted on cereals such as corn, rice, and wheat because | show 🗑
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| show | inhibiting an enzyme known as EPSP synthetase, which is required for aromatic amino acid production.
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| show 🗑
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| show | Bacillus thuringinensis.
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| show | Agrobacterium
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| show | make simple cuts across both DNA strands.
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| show | a recombinant DNA molecule
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| 44. Autonomous auxiliary DNA circles found in bacteria; easily enter the organism they are found in; used as a vehicle to transport desired foreign genes. | show 🗑
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| 45. Technique used to amplify a desired piece of DNA. | show 🗑
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| 46. Technique used to analyze DNA products after restriction enzyme digestion; "fingerprints." | show 🗑
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| show | transgenic organisms
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| 48. Ends of DNA fragments produced by asymmetric cleavage with restriction endonucleases; same ends are produced by the same enzyme irrespective of the source of the DNA. | show 🗑
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| show | 5' GATC 3'
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| show | 3
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| show | 1038 kb
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| show | 1038 kb, 3529 kb
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| show | phage vector
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| show | 43 white colonies, 6 blue colonies
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| 55. Your research project involves the characterization of a signal transduction pathway, and you would like to identify proteins that may interact with one of the pathway components. Which of the following libraries could you screen for this purpose? | show 🗑
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| 56. When compared with non-cancerous cells of the same tissue, cancer cells exhibit a number of changes in gene transcription. To identify genes that are differentially expressed in cancer cells versus noncancer cells, one could perform | show 🗑
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| 57. A researcher identified a new enzyme and is interested in determining the mRNA expression pattern of this gene. Which of the following techniques would accomplish this goal? | show 🗑
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| show | DNA fingerprinting
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| 59. Which of the following types of information would be most useful in an effort to move a DNA fragment from one plasmid vector to another, using molecular cloning techniques? | show 🗑
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| 60. Which of the following two enzymes are needed to construct a cDNA library | show 🗑
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| show | randomly at a single T position in the synthesized DNA strand, leading to termination of the reaction.
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| 62. Automated enzymatic sequencing employs | show 🗑
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| show | generation of hybrid plants
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| 64. You are performing a yeast two hybrid screen to identify protein-protein interactions. Your bait is a 25 kD protein fused to the GAL4 DNA-binding domain. Which of the following best describes the nature of the library that you are using? | show 🗑
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| show | introduce the knocked-out gene into embryonic stem cells, inject the embryonic stem cells into a blastocyst stage embryo, implant the injected blastocyst into a pseudopregnant female.
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| show | identification of the gene responsible for particular mutant phenotype
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| show | DNA vaccine.
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| 68. Current vectors used for gene therapy insert into the genome as a random event. All of the following are potential problems that may result from this method of integration except | show 🗑
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| 69. What of the following is a potential disadvantage of using transgenic plants for vaccine production? | show 🗑
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| 70. What is the environmental benefit of the Enviropig? | show 🗑
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| 71. Some food crops are difficult to grow for a variety of reasons. For example, strawberries are easily susceptible to frost damage. How could one use genetic engineering techniques help to overcome this type of problem, and which type of animal or plant | show 🗑
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Created by:
ClaEsc