Lab test #1
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| show | To avoid unnecessary exposure to potential pathogens.
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| Assuming you have a liquid microbial culture (broth culture) that you must dispose of. What are you expected to do with it? | show 🗑
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| While working in this labarotory, you will encounter a number of potentially harmful microorganisms. Aside from these, what are some of the hazards associated with work in the microbiology lab? | show 🗑
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| According to the CDC, handwashing is: | show 🗑
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| Did thoroughly scrubbing the hands reduce the number and variety of cells present? How do you know? | show 🗑
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| Did the data you obtained meet your expectations, or did the results differ from what you expected? | show 🗑
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| show | Compound or Bright-field microscope
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| The lens system nearest the specimen that magnifies and produces a real image is called ____, while the eyepiece lens system that magnifies the real image is called____. | show 🗑
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| show | revolving nosepiece / resolution
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| What objective do you use oil immersion on and why? | show 🗑
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| show | Course adjustment / fine adjustment
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| show | 4X (scanning) or 10X (low-power) / working distance is great enough that there is no danger of damaging a lens by forcing in into the glass of slides, coverslips or the substance condenser lens. It avoids damage to the prepared slides.
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| What is the significance of calibration? | show 🗑
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| show | The magnification of the object changes.
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| show | Broth media maintains its characteristic shape and arrangement Vs. solid media
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| show | Pellicle formation
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| show | Agar is a complex polysaccharide produced by marine algae. Most microorganisms do not use agar as a nutrient source, therefore it retains its solidifying effect despite metabolic activity.
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| In what ways do defined media differ from complex media? | show 🗑
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| Why are the culture media normally sterilized prior to use? | show 🗑
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| What is aseptic technique? When do we use it in this labarotory? | show 🗑
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| What is the function of flaming the mouths of tubes prior ro and following incubation? | show 🗑
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| show | To obtain one pure culture
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| show | To keep colonies forming from growing in one solid mass
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| Where are labels applied to plates containg microbial cultures and why? | show 🗑
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| What is a pure culture and what method is most commonly used to obtain a pure culture? | show 🗑
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| Which method of streak plate preperation (streak from a broth mixture or directly from a colony on agar) seemed to yeild the best result? What explanation can you give for the variation observed? | show 🗑
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| show | Carbon
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| What elements can bacteria pull from the air to make organic compunds? | show 🗑
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| What is the occular micrometer calibration for the 40X, 100X, 450X, and 1000X? | show 🗑
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| show | punctiform (pin-point), circular, irregular, filamentous, or rhizoid
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| show | Entire, undulate, lobate, serrate, filamentous, or curled
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| The elevation of a colony may be desribed as: | show 🗑
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| How do direct stains differ from inderect stains in terms of ionic composition and their interactions with cell surfaces? | show 🗑
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| Why is it important to form bacterial smears by mixing cells with a small amount of liquid and spreading them over a slide surface? | show 🗑
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| show | Heat-fixing makes the cells stick to the surface as well as kills the cells.
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| What is the cell wall made of? Is it found in all cell walls? What is its composition? | show 🗑
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| show | Gram-stain / this causes different cellls to look different
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| show | Gram-positive = thick peptidoglycan wall
Gram-negative = thin peptidoglycan wall
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| What is an important feature of the lipopolysaccharides (LPS) found in association with Gram-negative cell walls? | show 🗑
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| What is the function of a mordant? | show 🗑
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| show | There would be no color, making the cells unable to observe
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| During a KOH test what do you expect to see with a Gram-negative cell? | show 🗑
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| In what ways is the acid-fast stain similar to the Gram stain? | show 🗑
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| show | Wax-like lipid that causes resistance to staining.
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| show | No
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| show | nothing, it would remove the cells from the slide.
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| What color do acid-fast cella appear when stained with the acid-fast stain preperation? | show 🗑
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| show | dormant structures
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| What is a sporagium? | show 🗑
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| What is a glycocalyx? How do bacterial capsules and slime layers differ from one another? | show 🗑
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| show | Endospores typically appear as a white area within a darker colored cell, capsules usually have a white capsule surrounding a cell.
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| show | They increase the diameter making it visiable
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| show | Specific media or techniques used to promote the growth of certain organisms while inhibiting the growth of others.
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| Can you explain the enrichment procedures usedin this exercise to culture three different genera of bacteria from a single soil sample? | show 🗑
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| Were members of the genus Azotobacter the only types of bacteria observed to be growing on the nitrogen-free media? | show 🗑
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| show | No, because other organisms are able to survive off the same enrichment mediums.
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| What is bioluminescence and what enzymes are associated with this phenomenon? | show 🗑
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| show | They have amuch thicker outer membrane.
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| How do the cyanobacteria differ from most of the eubacteria you have been working with in lab? | show 🗑
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| show | No, they have fimbriae not flagella
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| show | Thread-like structures that make up the body or thallus of a mold type fungus
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| What are rhizoids? | show 🗑
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| show | hyphae that penetrate host cells and absorb nutrients.
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| show | specialized hyphae that help the plant obtain minerals and water from the soil.
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| What are conidiophores? | show 🗑
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| show | The slime molds (complex life cycles, resemble protozoa), flagellated lower fungi (live in water have walls of cellulose), and terrestrial fungi (mushrooms, fuff-balls, molds, yeast).
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| show | Acomycota
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| If you had a culture of fungi and bacteria, what selective plating procedure could you use to isolate each in a pure culture? | show 🗑
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| show | Dinoflagellata/ they have two flagella which beat within grooves, one transverse(running around the cell) the other lomgitudinal.
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| show | Unicellular: Chlamydomonas, desmids, diatoms, ceratium, peridiniumFilamentous: Spirogyra, ulothrix, oedogonium, cladaphora
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| show | Chlorophyta
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| show | Chemoheterotrophs / Euglenozoa
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| show | nuclei, Parabasal body (similar to golgi),flagella, pseudopodia, skeletons, cilia, cytosome
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| show | osmoregulation
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| Organisms called Trichnoympha are catergorized within which protozoan phylum? Where were these organisms found? | show 🗑
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| Which protozoa have more than one nucleus? | show 🗑
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| show | eukaryotic
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| What type of organisms fall into the catergorie generally identified as helminthes? | show 🗑
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| show | Flukes, tapeworms, and freeliving Planaria
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| What is an endoparasite; what is an ectoparasite, and which of the parasite listed falls into each catergory? | show 🗑
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Created by:
MichelleCheri