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Chapter 9

Quiz yourself by thinking what should be in each of the black spaces below before clicking on it to display the answer.
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Question
Answer
Process that destroys or removes all viable microorganisms (including viruses)   Sterilization  
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Physical process or a chemical agent to destroy vegetative pathogens but not bacterial endospores Removes harmful products of microorganisms (toxins) from material   Disinfection  
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Common uses of ______ include surgical instruments, syringes, commercially packaged food   Sterilization  
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Which concepts of antimicrobial control are normally restricted to inanimate objects?   Sterilization and disinfection  
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Common uses of ______ include boiling food utensils, applying 5% bleach solution to an examining table, immersing thermometers in an iodine solution between uses   Disinfection  
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Cleansing technique that mechanically removes microorganisms as well as other debris to reduce contamination to safe levels   Decontamination/Sanitization  
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Which concept of antimicrobial control is Important to restaurants, dairies, breweries, and other commercial entities that handle large numbers of soiled utensils/containers   Decontamination/Sanitization  
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Common uses of _____ include Cooking utensils, dishes, bottles, and cans for reuse.   Decontamination/Sanitization  
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Reduces the number of microbes on the human skin A form of decontamination but on living tissues   Antisepsis/degermation  
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Involves scrubbing the skin (mechanical friction) or immersing it in chemicals (or both)   Antisepsis/degermation  
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Heat (autoclave) Sterilants (chemical agents capable of destroying endospores) are examples of agents for ___________   Sterilization  
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Bleach Iodine Heat (boiling) are examples of agents for ____________   Disinfection  
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Soaps Detergents Commercial dishwashers are examples of agents for ___________   Decontamination/sanitization  
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Alcohol Surgical hand scrubs are examples of agents for _________   Antisepsis/degermation  
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The destruction or removal of vegetative pathogens but not bacterial endospores. Usually used only on inanimate objects.   Disinfection  
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The complete removal of destructions of all viable microorganisms. Used on inanimate objects.   Sterilization  
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Chemicals applied to body surfaces to destroy or inhibit vegetative pathogens.   Antisepsis/degermation  
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The mechanical removal of most microbes.   Decontamination/sanitization  
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_______ are extraordinarily resistant to heat and chemicals.   Prions  
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If objects become contaminated with _____, they must either be discarded as biohazards or, if this is not possible, a combination of chemicals and heat must be applied in accordance with the Centers for Disease Control and Prevention (CDC) guidelines.   Prions  
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_______ _______ of microbial control are microorganisms capable of causing infection or spoilage in the environment or on the human body.   Primary targets  
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The targeted population of contains mixtures of microbes with extreme differences in _______ and _________   resistance and harmfulness  
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_______ _________ have traditionally been considered the most resistant microbial entities.   Bacterial endospores  
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The goal of any sterilization process is the destruction of ______ _________   Bacterial endospores  
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Any process that kills ________ will invariably kill all less resistant microbial forms.   endospores  
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The root -_______, meaning “having the capacity to kill,” can be combined with other terms to define an antimicrobial agent aimed at destroying a certain group of microorganisms.   -cide  
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a _________ is a chemical that destroys bacteria except for those in the endospore stage. It may or may not be effective on other microbial groups.   bactericide  
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A ___________ is a chemical that can kill fungal spores, hyphae, and yeasts.   fungicide  
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A ________ is any chemical known to inactivate viruses, especially on living tissue.   varucide  
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A ___________ is an agent capable of destroying bacterial endospores.   sporicide  
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A _________ agent can also be a sterilant because it can destroy the most resistant of all microbes.   sporicidal  
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________ is defined as the growth of microorganisms in the blood and other tissues.   Sepsis  
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The term _________ refers to any practice that prevents the entry of infectious agents into sterile tissues and thus prevents infection.   Asepsis  
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__________ are applied directly to exposed body surfaces (skin and mucous membranes), wounds, and surgical incisions to destroy or inhibit vegetative pathogens.   Antiseptics  
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Examples of ___________ include preparing the skin before surgical incisions with iodine compounds, swabbing an open root canal with hydrogen peroxide, and ordinary hand washing with a germicidal soap.   Antisepsis  
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The Greek words stasis and static mean   To stand still  
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__________ can be used in combination with various prefixes to denote a condition in which microbes are temporarily prevented from multiplying but are not killed outright.   stasis and static  
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__________ agents prevent the growth of bacteria on tissues or on objects in the environment   Bacteriostatic  
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___________ chemicals inhibit fungal growth.   fungistatic  
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Materials used to control microorganisms in the body (antiseptics and drugs) often have ___________ effects because many microbicidal compounds can be highly toxic to human cells.   microbiostatic  
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A -cidal agent doesn't necessarily result in ___________, depending on how it is used.   sterilization  
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_________ medical devices are those that are expected to come into contact with sterile tissues, a syringe needle or an artificial hip.   Critical  
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_____________ devices are those that come into contact with mucosal membranes, such as an endoscopy tube.   Semicritical  
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Noncritical items are those that do not touch the patient or are only expected to touch intact skin, such as blood pressure cuffs or crutches.   Noncritical  
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Durable solids to sensitive liquids, situations requiring sterilization confront persons involved in health care are substances that require ___________   Sterilization  
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Cost, effectiveness, and method of disposal are all considerations for _________   Sterilization  
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__________ is permanent termination of an organism's vital processes   Death  
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Microbes have no conspicuous vital processes, therefore _______ is difficult to determine.   Death  
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Permanent loss of ____________ capability, even under optimum growth conditions has become the accepted microbiological definition of death.   reproductive  
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________ of the whole population is not instantaneous   Death  
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Death of the whole population begins when a certain threshold of ________ agent is met.   microbicidal  
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Death continues in a _________ manner as the time or concentration is increased.   logarithmic  
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Active cells tend to die more _______ than less metabolically active cells   quickly  
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Eventually, a point is reached at which survival of any cells is highly ________; this point is equivalent to _________   unlikely, sterilization  
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A higher load of contaminants takes ______ to destroy   longer  
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___________ __________ is usually a mixture of bacteria, fungi, spores, and viruses.   Target population  
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Temperature and ____ of the environment are factors affecting the death rate.   pH  
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UV radiation is most effective at _______ nm   260  
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Most disinfectants are more active at __________ concentrations.   higher  
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The mode of _______ of the agent is a factor affecting death.   action  
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_________, ________, and ______ can inhibit the action of disinfectants and even the action of heat.   saliva, blood, and feces  
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________ selective agents tend to be effective against the widest range of microbes (heat and radiation).   Least  
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__________ agents target only a single cellular component (drugs).   agents  
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The cell wall, cell membrane, cellular synthetic processes, and proteins are all considered to be cellular _______ of physical and chemical agents.   targets  
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____________ effects on the cell wall block its synthesis, digest it, break down its surface, and the cell becomes fragile and is lysed easily.   Antimicrobial  
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Which microorganisms have a cell membrane?   They all do  
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If the cell membrane is disrupted, the cell loses the ability to be ________ ________   selectively permeable  
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__________ disrupt cell membranes   Detergents (surfactants)  
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Which level of protein or nucleic acid synthesis can be affected?   Replication, transcription, and/or translation  
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Some agents might bind to _________ to stop translation   ribosomes  
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Some agents bind irreversibly to _______ preventing transcription and translation   DNA  
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Agents that can damage the cell wall include ________, _________, and ______   chemicals, detergents, and alcohol  
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Agents that can damage the cytoplasmic membrane include _________   Detergents  
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Agents that can interrupt cellular synthesis include _________, ________, and ________ ________   Formaldehyde, radiation, and ethylene oxide  
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Agents that can denature proteins include __________ __________, ____________, and _________   Moist heat, alcohol, and phenolics  
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Chemical agents can damage the cell wall through which two methods?   blocking its synthesis or digesting the cell wall  
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Chemical agents can physically bind to the lipid layer of the _________ ________, opening up the membrane and allowing injurious chemicals to enter the cell and important ions to exit the cell.   cytoplasmic membrane  
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Chemical agents can interrupt the ________ of proteins as well as change ________ _________.   synthesis, genetic code  
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The use of iodine compounds to prepare the skin for surgery is known as A. disinfection. B. antisepsis. C. sterilization. D. sanitization. E. degermation.   A. disinfection  
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Elevated temperatures are __________   microbicidal  
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Lower temperatures are __________   microbistatic  
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________ heat is hot water, boiling water, or steam between 60°C and135°C   moist  
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________ heat is hot air or an open flame, which ranges from 160°C to thousands of degrees Celsius   dry  
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Heat is a method of physical _________   control  
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________ heat operates at lower temperatures and shorter exposure times to achieve the same effectiveness as dry heat   Moist  
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Moist heat's microbicidal effect is the ___________ and _________ of proteins   coagulation and denaturation  
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________ heat dehydrates the cell, removing water necessary for metabolic reactions   dry  
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Bacterial ________ exhibit great, varying, resistance and destruction usually requires temperatures above boiling.   Endospores  
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_______ cells vary in their sensitivity to heat and death times vary from 50°C for 3 minutes to 60°C for 60 minutes   Vegetative  
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Fungi, protozoa, and ________ are similar in their sensitivity to heat.   worms  
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_______ have a heat tolerance that extends from 55°C for 2 – 5 minutes to 60°C for 600 minutes.   viruses  
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_______ ________ _________ is the shortest length of time required to kill all test microbes at a specified temperature   Thermal death time (TDT)  
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_______ ___________ _________ is the the lowest temperature required to kill all microbes in a sample in 10 minutes   Thermal death point  
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_______ ______ disinfection is useful in the home for disinfection of water, materials for babies, food and utensils, bedding, and clothing from the sickroom   boiling water  
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___________ is a technique in which heat is applied to liquids to kill potential agents of infection and spoilage, while at the same time retaining the liquid’s flavor and food value.   Pasteurization  
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