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Quiz yourself by thinking what should be in each of the black spaces below before clicking on it to display the answer.
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Term
Definition
Magnification   The degree to which the image of a specimen is enlarged.  
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Resolution   The ability to distinguish individual features as separate; the ability to see detail. Typically,as magnification increases it increases.  
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Contrast   How well features stand out against the background. Magnification does not affect it. To improve it a biological stain must be used. (Blue idion -plant tissue)  
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Field of view   The area seen when looking through the ocular lens. As magnification increases, it decreases....the circle visible through the lenses.  
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Diameter of field   The measure length of the field view.  
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Depth of focus/field/view   The vertical distance always in focus with a particular objective lens. As magnification increases, it decreases.  
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Working distance   The vertical distance from the specimen to the objective lens when focused. As magnification increases, it decreases.  
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Parfocal   The ability to change magnification and keep the image focused.  
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Parcentral   The ability to change magnification and the image centered in the field of view.  
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Compound Light Microscope (lens & magnification)   Ocular lens or eyepiece (10x) Scanning objective lens.(4x) Low power objective lens (10x) High-def objective lens. (40x) Oil immersion objective lens (100x)  
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Stereo/dissecting microscope (lens & magnification)   Ocular (1x) 10x objective 20x objective  
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2 types of microscopy   1. Electron microscopy 2. Light microscopy  
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2 types of electronic microscopes   Scanning electron microscope- reflects the beam off the surface of the specimen and is therefore useful for observing surface features. Transmission electron microscope- Transmits the electron beam through the specimen and is useful for observing internal  
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Compound Light Microscope   Uses more than one lens...results in inversion....use transmitted light which pass through the specimen...useful in observing internal features.  
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Stereo/dissecting microscope   Uses single length to produce magnified image so inversion doesn't occur....uses reflected light which bounced off the surface of the specimen....useful for observing surface features.  
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Ocular lens (eyepiece)   10x  
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Viewing head   Hold the ocular lenses  
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Arm   Support upper parts and provides carrying handle.  
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Nose piece   Revolving device that holds objective.  
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Scanning objective len   The shortest...used to scan the whole slide...magnifying power 4x  
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Low-power objective lens   Used to view objects in greater details....magnifying power 10x  
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High-power objective lens   Largest of scanning and low-power lens....used to view greater details ...magnifying power 40x  
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Oil - immersion objective lens   Holds a 95x to 100x lens...used in conjunction with immersion oil to view objects with the greatest magnification  
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Stage   Holds & supports microscope slides. Mechanics one is movable  
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Coarse - adjustable knob   Used to bring object into approximate focus;..used only with low powered objective  
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Fine-adjustment knob   Used to bring object into final focus  
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Condenser   Lens system below stage used to focus the beam of light on the object being viewed  
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Diaphragm or diaphragm control lever   Controls the amount of illumination used to view the object.  
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Light source   An attached lamp that directs a beam of light up through the object.  
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Base   The flat surface of the microscope that rests on the table.  
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