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Magnification The degree to which the image of a specimen is enlarged.
Resolution The ability to distinguish individual features as separate; the ability to see detail. Typically,as magnification increases it increases.
Contrast How well features stand out against the background. Magnification does not affect it. To improve it a biological stain must be used. (Blue idion -plant tissue)
Field of view The area seen when looking through the ocular lens. As magnification increases, it decreases....the circle visible through the lenses.
Diameter of field The measure length of the field view.
Depth of focus/field/view The vertical distance always in focus with a particular objective lens. As magnification increases, it decreases.
Working distance The vertical distance from the specimen to the objective lens when focused. As magnification increases, it decreases.
Parfocal The ability to change magnification and keep the image focused.
Parcentral The ability to change magnification and the image centered in the field of view.
Compound Light Microscope (lens & magnification) Ocular lens or eyepiece (10x) Scanning objective lens.(4x) Low power objective lens (10x) High-def objective lens. (40x) Oil immersion objective lens (100x)
Stereo/dissecting microscope (lens & magnification) Ocular (1x) 10x objective 20x objective
2 types of microscopy 1. Electron microscopy 2. Light microscopy
2 types of electronic microscopes Scanning electron microscope- reflects the beam off the surface of the specimen and is therefore useful for observing surface features. Transmission electron microscope- Transmits the electron beam through the specimen and is useful for observing internal
Compound Light Microscope Uses more than one lens...results in inversion....use transmitted light which pass through the specimen...useful in observing internal features.
Stereo/dissecting microscope Uses single length to produce magnified image so inversion doesn't occur....uses reflected light which bounced off the surface of the specimen....useful for observing surface features.
Ocular lens (eyepiece) 10x
Viewing head Hold the ocular lenses
Arm Support upper parts and provides carrying handle.
Nose piece Revolving device that holds objective.
Scanning objective len The shortest...used to scan the whole slide...magnifying power 4x
Low-power objective lens Used to view objects in greater details....magnifying power 10x
High-power objective lens Largest of scanning and low-power lens....used to view greater details ...magnifying power 40x
Oil - immersion objective lens Holds a 95x to 100x lens...used in conjunction with immersion oil to view objects with the greatest magnification
Stage Holds & supports microscope slides. Mechanics one is movable
Coarse - adjustable knob Used to bring object into approximate focus;..used only with low powered objective
Fine-adjustment knob Used to bring object into final focus
Condenser Lens system below stage used to focus the beam of light on the object being viewed
Diaphragm or diaphragm control lever Controls the amount of illumination used to view the object.
Light source An attached lamp that directs a beam of light up through the object.
Base The flat surface of the microscope that rests on the table.
Created by: 1394349