Busy. Please wait.
Log in with Clever
or

show password
Forgot Password?

Don't have an account?  Sign up 
Sign up using Clever
or

Username is available taken
show password

Your email address is only used to allow you to reset your password. See our Privacy Policy and Terms of Service.


Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.

HTL Nuc/cytop stains

Quiz yourself by thinking what should be in each of the black spaces below before clicking on it to display the answer.
        Help!  

Question
Answer
show bound chemically as opposed to "soaked up"  
🗑
define absorbtion   show
🗑
show substances, often metals, that act as a link between the dye and the tissue  
🗑
define basophilia   show
🗑
show acid loving. Substances that attract/are attracted to acid dyes  
🗑
define ripening   show
🗑
define auxochrome   show
🗑
define chromophore   show
🗑
define polychrome   show
🗑
show A basic dye. One where the charge of the dye ion is positive. the auxochrome is the amino group NH2  
🗑
define anionic dye   show
🗑
define dye lake   show
🗑
describe and differentiate heterochromatin and euchromatin   show
🗑
how can cell activity affect H&E staining   show
🗑
differentiate hematin and hematein   show
🗑
define progressive and regressive staining   show
🗑
show Harris, Gill, Mayer  
🗑
show Delafield, Ehrlich, weigert  
🗑
list 3 methods of differentiation   show
🗑
list 4 natural dyes and their sources   show
🗑
show Mordant: aluminum. Oxidizer (ripening): natural or chemical. commonly regressive, can be used progressively.  
🗑
show Mordant: aluminum. Oxidizer (ripening): natural. Stabilized with glycerol against overoxidation and evaporation. Intended as regressive stain.  
🗑
describe Harris hematoxylin   show
🗑
describe Mayer hematoxylin   show
🗑
describe Gill hematoxylin   show
🗑
describe Weigert hematoxylin   show
🗑
show To resist decolorization in acidic solutions  
🗑
how would excess acid or aluminum effect hematoxylin staining   show
🗑
how does pH effects H&E staining?   show
🗑
show temperature, dye concentration, salts other than the dye itself in solution  
🗑
show They will lose their basophilia (nuclear staining)  
🗑
how do formalin and zenkers affect staining   show
🗑
ID 6 sources of error in H&E   show
🗑
ID 2 types of nucleic acids and 2 methods to stain them   show
🗑
what specific substances are stained in nuclear stains   show
🗑
describe acid hydrolysis and fixation for Feulgen reactions   show
🗑
show Hematoxylin, celestine blue, nuclear-fast red, methylene blue, thionin, toluidine blue O  
🗑
describe the romanowski stains and their two uses   show
🗑
what are 2 classes of mounting media   show
🗑
give advantages of each media   show
🗑
show Nail polish, glue or other cement. this will preserve the aqueous mounting media against evaporation, smearing, etc. so that the slide may be stored in a more permanent fashion.  
🗑
show Natural resins are inherently acidic and will cause fading of some stains. They also tend to yellow with age, and may take days or months to set completely. Synthetic resins solve all of these problems.  
🗑
show the thicker the coverslip the less transparent the section will be.  
🗑
show Resinous= 1.51-1.55 Aqueous= 1.41-1.43 Tissue= ~1.53-1.54  
🗑
coverslipping artifacts   show
🗑
show Cornflaking (sections dried before mounting) Water droplets (incomplete dehydration) retracted media (too thin at application)  
🗑
Define amphoteric   show
🗑
show COOH, OH, SO3H  
🗑
What is a common basic auxochrome?   show
🗑
define adjective staining   show
🗑
show a stain where the dye acts directly with the tissue, and does not require another substance  
🗑
define impregnation   show
🗑
show to demonstrate nuclei via hydrolysis of sugars to aldehydes and PAS  
🗑
show HCl --> DIH2O --> Shiffs --> water --> Sulfurous acid --> light green (option)  
🗑
show demonstration of DNA and RNA, respectively  
🗑
what are the reagents used in methyl green pyronin Y reaction?   show
🗑


   

Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
 
To hide a column, click on the column name.
 
To hide the entire table, click on the "Hide All" button.
 
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
 
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.

 
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how
Created by: joshuajohnson7