HTL Nuc/cytop stains
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show | bound chemically as opposed to "soaked up"
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define absorbtion | show 🗑
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show | substances, often metals, that act as a link between the dye and the tissue
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define basophilia | show 🗑
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show | acid loving. Substances that attract/are attracted to acid dyes
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define ripening | show 🗑
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define auxochrome | show 🗑
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define chromophore | show 🗑
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define polychrome | show 🗑
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show | A basic dye. One where the charge of the dye ion is positive. the auxochrome is the amino group NH2
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define anionic dye | show 🗑
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define dye lake | show 🗑
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describe and differentiate heterochromatin and euchromatin | show 🗑
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how can cell activity affect H&E staining | show 🗑
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differentiate hematin and hematein | show 🗑
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define progressive and regressive staining | show 🗑
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show | Harris, Gill, Mayer
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show | Delafield, Ehrlich, weigert
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list 3 methods of differentiation | show 🗑
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list 4 natural dyes and their sources | show 🗑
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show | Mordant: aluminum. Oxidizer (ripening): natural or chemical. commonly regressive, can be used progressively.
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show | Mordant: aluminum. Oxidizer (ripening): natural. Stabilized with glycerol against overoxidation and evaporation. Intended as regressive stain.
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describe Harris hematoxylin | show 🗑
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describe Mayer hematoxylin | show 🗑
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describe Gill hematoxylin | show 🗑
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describe Weigert hematoxylin | show 🗑
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show | To resist decolorization in acidic solutions
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how would excess acid or aluminum effect hematoxylin staining | show 🗑
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how does pH effects H&E staining? | show 🗑
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show | temperature, dye concentration, salts other than the dye itself in solution
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show | They will lose their basophilia (nuclear staining)
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how do formalin and zenkers affect staining | show 🗑
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ID 6 sources of error in H&E | show 🗑
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ID 2 types of nucleic acids and 2 methods to stain them | show 🗑
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what specific substances are stained in nuclear stains | show 🗑
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describe acid hydrolysis and fixation for Feulgen reactions | show 🗑
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show | Hematoxylin, celestine blue, nuclear-fast red, methylene blue, thionin, toluidine blue O
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describe the romanowski stains and their two uses | show 🗑
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what are 2 classes of mounting media | show 🗑
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give advantages of each media | show 🗑
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show | Nail polish, glue or other cement. this will preserve the aqueous mounting media against evaporation, smearing, etc. so that the slide may be stored in a more permanent fashion.
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show | Natural resins are inherently acidic and will cause fading of some stains. They also tend to yellow with age, and may take days or months to set completely. Synthetic resins solve all of these problems.
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show | the thicker the coverslip the less transparent the section will be.
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show | Resinous= 1.51-1.55
Aqueous= 1.41-1.43
Tissue= ~1.53-1.54
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coverslipping artifacts | show 🗑
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show | Cornflaking (sections dried before mounting) Water droplets (incomplete dehydration) retracted media (too thin at application)
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Define amphoteric | show 🗑
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show | COOH, OH, SO3H
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What is a common basic auxochrome? | show 🗑
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define adjective staining | show 🗑
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show | a stain where the dye acts directly with the tissue, and does not require another substance
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define impregnation | show 🗑
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show | to demonstrate nuclei via hydrolysis of sugars to aldehydes and PAS
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show | HCl --> DIH2O --> Shiffs --> water --> Sulfurous acid --> light green (option)
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show | demonstration of DNA and RNA, respectively
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what are the reagents used in methyl green pyronin Y reaction? | show 🗑
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joshuajohnson7