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Microbiology

Quiz yourself by thinking what should be in each of the black spaces below before clicking on it to display the answer.
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Question
Answer
show both phage DNA and bacterial DNA integrate into the chromosome of the new cell host  
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In the process of specialized transduction   show
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show only bacterial genes near the site of integration of the phage DNA can be transduced  
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The lambda phage DNA always integrates into the host DNA in the same specific site.   show
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show False  
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show Temperate virus  
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Ribotyping   show
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show Flurorescent In Situ Hybridization, do NOT need to culture colonies  
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show it selectively allows some molecules to pass into the organism it prevents movement of molecules out of the organism it is the site of protein synthesis  
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show coccus  
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show Site of protein synthesis  
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Plasmids are important to the genetics of many bacteria. This is because   show
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Bacteria do not always swim aimlessly but are attracted by such nutrients such as sugar and amino acids, and are repelled by harmful substances and bacterial waste products. Movement toward chemical attractants and away from repellents is called   show
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show help move cells to optimal nutrient levels.  
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Gram positive cells   show
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The presence of D-amino acids in the crosslinks of the peptidoglycan layer is most likely because   show
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The third amino acid in the peptidoglycan crosslinking chain is either diaminopimilic acid or lysine because this amino acid must   show
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show do not have an outer membrane. have large amounts of teichoic acids have thick, homogeneous cell walls.  
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show Clostridium difficile Carbapenem-resistant Enterobacteriaceae (CRE) Drug-resistant Neisseria gonorrhoeae  
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show porin proteins establish holes in the outer membrane.  
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The most important role of the prokaryotic cell wall is to   show
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show Allow the bacterium to survive extended periods of heat or dryness.  
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Serious threats   show
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show Vancomycin-resistant Staphylococcus aureus (VRSA) Erythromycin-resistant Group A Streptococcus  
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WHERE DO INFECTIONS HAPPEN?   show
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show 250,000 ilnesses 14,000 deaths  
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show 2,049,442 illnesses 23,000 deaths  
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Animal to human antibiotic resistance   show
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show Resistant bacteria spread to other patients from surfaces within the healthcare facility.  
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show Simply using antibiotics creates resistance. These drugs should only be used to treat infections  
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show L-Lysine cross links to D-alanine in G+  
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show True; Archaea have monolayer, bacteria have bilayer  
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which part of the bilayer is hydrophobic   show
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what makes up the hydrophilic portion of the bilayer   show
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show Today, the international identification of antibiotic resistance threats occurs through domestic importation of novel antibiotic resistance threats or through identification of overseas outbreaks  
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show Advanced molecular detection (AMD) technologies, which can identify AR threats much faster than current practice, are not being used as widely as necessary in the United States  
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PREVENTING INFECTIONS, PREVENTING THE SPREAD OF RESISTANCE...Avoiding infections in the first place reduces the amount of antibiotics that have to be used and reduces the likelihood that resistance will develop during therapy.   show
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show CDC gathers data on antibiotic-resistant infections, causes of infections and whether there are particular reasons (risk factors) that caused some people to get a resistant infection.  
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show s the single most important action needed to greatly slow down the development and spread of antibiotic-resistant infections is to change the way antibiotics are use  
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show Because antibiotic resistance occurs as part of a natural process in which bacteria evolve, it can be slowed but not stopped.  
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show 6-8 nanometers wide  
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show Mg2+ and Ca2+ ions stabilize membranes by forming ionic bonds with negative charges on phospholipids  
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what is unique about Archaeal membranes?   show
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What does the cell membrane do?   show
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show NAM (N-Acetylmuramic acid) NAG (N-Acetylglucosamine)  
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show L-alanine D-glutamic acid If G-, DAP If G+, Lysine D-alanine  
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What is the configuration of beta 1-4 linkage for NAG/NAM?   show
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show D-glu stands for D-glutamic acid  
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show KDO 7 carbon sugar Glucose, galactose, NAG  
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show O-specific lipopolysaccharide Core polysaccharide Lipid A  
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show Housekeeping genes. Has sufficient resolving power to distinguish between very closely related strains.  
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show protocells…came from subsurface ocean near thermal vents. Clay played role in being a solid substrate for rxns to occur  
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SSrna strands are good candidates for phylogenetic analysis because   show
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Most widely used molecular clocks are   show
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show Bacteria have at least 80 unique lineages…largest group is the proteobacteria, which shows all types of microbial physiology.  
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Archaea consist mostly of   show
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Endosymbiosis theory   show
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Mitochondria/ Chloroplasts support endosymbiosis theory how?   show
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show Gene loss is common among obligate symbionts and parasites  
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show 97% or greater similarity in ss rRNA = same species.  
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FAME= Fatty Acid Methyl Ester   show
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show Examines the DNA of two closely related species and looks at where the DNA matches up. Greater than 70% means same species, greater than 25% means same genus.  
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prokaryotic species is currently defined as   show
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many groups are phenotypically diverse...   show
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Mitochondria arose from __________   show
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show chloroplasts arose from cyanobacteria.  
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show REC A protein= KEY TO HOMOLOGOUS RECOMBINATION (identified in ALL domains of life)  
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show Lytic cycle= when viruses get into cell and force cell to make more viruses, eventually lysing the bacterial cell. Virus sis a transducing agent since it contains some of the host DNA. Cell gets transduced.  
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show Viral DNA pastes into host DNA making the virus a prophage, the cell is infected but does not produce more viruses. Viral DNA has to get cut out to be activated.  
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show power in numbers. Genes on a plasmid can alter cell phenotype, leads to selective advantage in habitat. Plasmid transfer= highly efficient, can transmit between distantly related species  
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show DNA from a specific region of the host chromosomes is integrated directly into the virus genome, usually replacing viral genes. ONLY HAPPENS IN TEMPERATE PHAGES. Homologous recombination may occur.  
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Transducing particle:   show
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Rolling circle replication   show
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show Lineage: Bacteria to fungi Significance: Adaption to insect environment  
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Gene: Multiple genes   show
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show Lineage: Bacteria to fungi Significance: Adaption to rumen environments  
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show 0.5 to 2 micrometers is the general size of bacteria  
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Biggest bacteria?   show
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why are small cells better than large cells   show
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Why do mutations appear so rapidly   show
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Hopanoids=   show
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What is unique about archaeal lipids   show
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Bacteria and eukarya are held together by __________ ___________   show
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show Phospholipids and lipoproteins, which anchors the outer membrane to peptidoglycan  
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show Channel proteins hydrphilic, low molecular weight molecules flow through  
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FeS + H2S --> FeS2 + H2 = -42kJ.   show
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What is the NAG/NAM repeating unit called?   show
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Do gram negative bacteria have interbridges in their peptidoglycan sheet?   show
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show Space between inner surface of OM and outer surface of CM. It is gel like, and contains Hydrolytic enzymes, Binding proteins, and Chemoreceptors  
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show 12-15 nanometers  
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show Made of Pseudopeptidoglycan, which has the two sugar derivatives NAG + NAT, Linkage of b-1,3 (not b-1,4)  
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What is the Paracrystalline surface layer (S-layer)   show
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why is it important to know what kind of infection you have before you take antibiotics   show
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show H2 are actually protons, their electrons have been stripped away to be used as fuel, resulting in an accumulation of H+ protons outside of the cell(concentration gradient). As protons flow through ATP synthase torque is created. result is ADP +Pi-->ATP  
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What is pseudopeptidoglycan   show
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What types of cell wall can archaea have   show
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show paracrystalline surface layer (>50%)  
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what does B-1-3 lysozyme insensitive mean   show
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show lysozyme effectively attacks and breaks down the peptidoglycan of bacterial cells.  
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show 50 genes need to be expressed to make a flagella.  
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show Fli proteins-function as motor switch, flagellin proteins, mot proteins cause torque  
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show Motor portion= anchored in cell wall..ring system: L-ring, p-ring, ms-ring, c-ring.  
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How many protons does it take for one rotation   show
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show mot protein causes torque; need proton gradient to do so. proton flow causes torque.  
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What connects the motor to the filament   show
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Peritrichous   show
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show single flagella at one end  
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show numerous flagella at one end  
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What is required for gliding motility?   show
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show response to chemicals  
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show Phototaxis: response to light  
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Aerotaxis   show
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show Osmotaxis: response to ionic strength  
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show Hydrotaxis: response to water  
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show extracellular polymeric material produced by some bacteria, Capsule & Slime Layers Polysaccharide or protein  
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What is gliding motility?   show
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show Gliding motility requires gliding-specific proteins, type four pili, and polysaccharide slime  
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show The glycocalyx produces biofilms, assists in adherence, assists in nutrient collection, helps resist dessication, and helps evade phagocytosis.  
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