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Specimen Collection and Quality Control

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Question
Answer
What does an accurate chemical analysis depend on?   Proper collection, preservation, processing, method of analysis  
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What is the ideal time for lab measurements to be performed?   1 Hour or remove serum from clot within 2 hours.  
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Why are specimens preserved?   sample retained long enough to repeat if necessary.  
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What sample is most often used for chemical analysis?   Serum (fasting), no food/bev. other than water and meds for at least 12 hours.  
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What is plasma?   Liquid portion of the circulating blood, contains fibrinogen and clotting factors.  
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What is serum?   Liquid portion of blood with fibrinogen and clotting factors removed. (Gold/Red Plastic Tubes)  
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What is the Thixotropic gel used for?   Separates Serum or plasma from the cells, prevents shifts of substances and prevents Glycolysis.  
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What Vacutainer is used to collect serum and why   Gold or Red/Grey or Red Tubes. Serum not plasma no anticoagulants.  
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How is a gold top tube processed?   Invert 5 times, do not remove stopper , Do not centrifuge immediately after drawing.  
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How long should a Gold Top tube sit before centrifuging?   allow to clot upright, 30 but not more than 60 minutes, Centrifuge at least 10-15 minutes.  
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How long should a Transport tube be centrifuged?   Transport tube (Red/Grey) has extra gel inside and should be centrifuged for 15 minutes.  
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Hemolyzed Serums   (plasma). Liquid is a clear/red color  
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Icteric (jaundiced, hyper bilirubinemia)   Liquid portion of blood is brownish/yellow  
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Lipemic   Liquid portion of blood/serum is milky-white. (lipids)  
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Accuracy   True or actual Value  
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Precision   Repeatability (ability to get same answer again)  
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Normal Value   The average result of Healthy individuals plus or minus 2 Standard deviations.  
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Batch or Run   specimens analyzed at the same time  
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Calibrator   used to set machine values  
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Control   must fall within acceptable range before patient values can be reported.  
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What four things does the control check for?   Reagents, Instrument, operator, environment (temp/humidity)  
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What steps should you follow if a control falls outside of the acceptable range?   check everything, retest same control vial, new vial of control, recalibrate, call customer service as a last resort  
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Important things about Controls   Made from human blood products, 2 expiration dates, one open the other not, open varies depending on Control, acceptable range published by manuf.  
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duplicate Determination   measure one specimen in a batch twice  
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CLIA '88   Regulations for Labs,  
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Non-Waived Tests,High Complexity, Moderately Waived   subjected to more regulations  
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Waived   exempted from most regulations, results are not life threatening, must remember to do Q/C as specified by kit manuf. and keep Q/C records for 2 years,.  
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