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Specimen Collection
Specimen Collection and Quality Control
| Question | Answer |
|---|---|
| What does an accurate chemical analysis depend on? | Proper collection, preservation, processing, method of analysis |
| What is the ideal time for lab measurements to be performed? | 1 Hour or remove serum from clot within 2 hours. |
| Why are specimens preserved? | sample retained long enough to repeat if necessary. |
| What sample is most often used for chemical analysis? | Serum (fasting), no food/bev. other than water and meds for at least 12 hours. |
| What is plasma? | Liquid portion of the circulating blood, contains fibrinogen and clotting factors. |
| What is serum? | Liquid portion of blood with fibrinogen and clotting factors removed. (Gold/Red Plastic Tubes) |
| What is the Thixotropic gel used for? | Separates Serum or plasma from the cells, prevents shifts of substances and prevents Glycolysis. |
| What Vacutainer is used to collect serum and why | Gold or Red/Grey or Red Tubes. Serum not plasma no anticoagulants. |
| How is a gold top tube processed? | Invert 5 times, do not remove stopper , Do not centrifuge immediately after drawing. |
| How long should a Gold Top tube sit before centrifuging? | allow to clot upright, 30 but not more than 60 minutes, Centrifuge at least 10-15 minutes. |
| How long should a Transport tube be centrifuged? | Transport tube (Red/Grey) has extra gel inside and should be centrifuged for 15 minutes. |
| Hemolyzed Serums | (plasma). Liquid is a clear/red color |
| Icteric (jaundiced, hyper bilirubinemia) | Liquid portion of blood is brownish/yellow |
| Lipemic | Liquid portion of blood/serum is milky-white. (lipids) |
| Accuracy | True or actual Value |
| Precision | Repeatability (ability to get same answer again) |
| Normal Value | The average result of Healthy individuals plus or minus 2 Standard deviations. |
| Batch or Run | specimens analyzed at the same time |
| Calibrator | used to set machine values |
| Control | must fall within acceptable range before patient values can be reported. |
| What four things does the control check for? | Reagents, Instrument, operator, environment (temp/humidity) |
| What steps should you follow if a control falls outside of the acceptable range? | check everything, retest same control vial, new vial of control, recalibrate, call customer service as a last resort |
| Important things about Controls | Made from human blood products, 2 expiration dates, one open the other not, open varies depending on Control, acceptable range published by manuf. |
| duplicate Determination | measure one specimen in a batch twice |
| CLIA '88 | Regulations for Labs, |
| Non-Waived Tests,High Complexity, Moderately Waived | subjected to more regulations |
| Waived | exempted from most regulations, results are not life threatening, must remember to do Q/C as specified by kit manuf. and keep Q/C records for 2 years,. |
Created by:
charndoug
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