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Specimen Collection and Quality Control
|What does an accurate chemical analysis depend on?
|Proper collection, preservation, processing, method of analysis
|What is the ideal time for lab measurements to be performed?
|1 Hour or remove serum from clot within 2 hours.
|Why are specimens preserved?
|sample retained long enough to repeat if necessary.
|What sample is most often used for chemical analysis?
|Serum (fasting), no food/bev. other than water and meds for at least 12 hours.
|What is plasma?
|Liquid portion of the circulating blood, contains fibrinogen and clotting factors.
|What is serum?
|Liquid portion of blood with fibrinogen and clotting factors removed. (Gold/Red Plastic Tubes)
|What is the Thixotropic gel used for?
|Separates Serum or plasma from the cells, prevents shifts of substances and prevents Glycolysis.
|What Vacutainer is used to collect serum and why
|Gold or Red/Grey or Red Tubes. Serum not plasma no anticoagulants.
|How is a gold top tube processed?
|Invert 5 times, do not remove stopper , Do not centrifuge immediately after drawing.
|How long should a Gold Top tube sit before centrifuging?
|allow to clot upright, 30 but not more than 60 minutes, Centrifuge at least 10-15 minutes.
|How long should a Transport tube be centrifuged?
|Transport tube (Red/Grey) has extra gel inside and should be centrifuged for 15 minutes.
|(plasma). Liquid is a clear/red color
|Icteric (jaundiced, hyper bilirubinemia)
|Liquid portion of blood is brownish/yellow
|Liquid portion of blood/serum is milky-white. (lipids)
|True or actual Value
|Repeatability (ability to get same answer again)
|The average result of Healthy individuals plus or minus 2 Standard deviations.
|Batch or Run
|specimens analyzed at the same time
|used to set machine values
|must fall within acceptable range before patient values can be reported.
|What four things does the control check for?
|Reagents, Instrument, operator, environment (temp/humidity)
|What steps should you follow if a control falls outside of the acceptable range?
|check everything, retest same control vial, new vial of control, recalibrate, call customer service as a last resort
|Important things about Controls
|Made from human blood products, 2 expiration dates, one open the other not, open varies depending on Control, acceptable range published by manuf.
|measure one specimen in a batch twice
|Regulations for Labs,
|Non-Waived Tests,High Complexity, Moderately Waived
|subjected to more regulations
|exempted from most regulations, results are not life threatening, must remember to do Q/C as specified by kit manuf. and keep Q/C records for 2 years,.