Final Exam Questions
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| What governs the distribution of the solutes between the aqueous phase and the organic phase? | Their relative solubilities
🗑
|
||||
| How can one ascertain whether a layer is the organic phase or the aqueous phase? | Add a drop of the mystery layer to 2 mL H2O; if it dissolves, it's aqueous; if not, it's organic
🗑
|
||||
| Why must extraction mixtures be vented? | Organic solvents are volatile and evaporate quickly, generating pressure inside the separatory funnel
🗑
|
||||
| What three advantages are associated with the thin-layer chromatographic technique? | 1) very small sample quantities are required, 2) great ease and rapidity with which experiment's performed, 3) good resolution's often obtained
🗑
|
||||
| What two limitations are associated with gas-liquid chromatography? | 1) analysis is limited to small amounts of sample, 2) samples must consist of reasonably volatile compounds
🗑
|
||||
| What are the six basic parts of a gas-liquid chromatograph? | 1) source of high-pressure pure carrier gas (e.g., helium or nitrogen), 2) gas flow controller, 3) heated injection port, 4) column and column oven, 5) detector, 6) recording device or data station
🗑
|
||||
| What three pieces of information are provided in the data table of a gas chromatogram? | 1) peak retention times, 2) peak areas, 3) percent compositions
🗑
|
||||
| What must be available in order for GC analysis to permit compound identification? | Authentic samples of the various components in the mixture being analyzed
🗑
|
||||
| What are the six items that must be determined in order to establish the sequence of a peptide (or protein)? | 1) cleave all disulfide bridges and alkylate resulting thiol groups, 2) determine constituent amino acids, 3) identify N-terminal aa, 4) identify C-terminal aa, 5) fragmentation through partial hydrolysis, 6) identify locations of any disulfide bridges
🗑
|
||||
| What conditions are employed to "completely" hydrolyze a peptide? | Digest peptide in 6 M HCl at 100 degrees C for 24 hours
🗑
|
||||
| An enzyme that catalyzes the hydrolysis of a peptide bond at the end of the peptide chain is called? | Exopeptidase
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
knye
Popular Chemistry sets