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CH 337

Final Exam Questions

What governs the distribution of the solutes between the aqueous phase and the organic phase? Their relative solubilities
How can one ascertain whether a layer is the organic phase or the aqueous phase? Add a drop of the mystery layer to 2 mL H2O; if it dissolves, it's aqueous; if not, it's organic
Why must extraction mixtures be vented? Organic solvents are volatile and evaporate quickly, generating pressure inside the separatory funnel
What three advantages are associated with the thin-layer chromatographic technique? 1) very small sample quantities are required, 2) great ease and rapidity with which experiment's performed, 3) good resolution's often obtained
What two limitations are associated with gas-liquid chromatography? 1) analysis is limited to small amounts of sample, 2) samples must consist of reasonably volatile compounds
What are the six basic parts of a gas-liquid chromatograph? 1) source of high-pressure pure carrier gas (e.g., helium or nitrogen), 2) gas flow controller, 3) heated injection port, 4) column and column oven, 5) detector, 6) recording device or data station
What three pieces of information are provided in the data table of a gas chromatogram? 1) peak retention times, 2) peak areas, 3) percent compositions
What must be available in order for GC analysis to permit compound identification? Authentic samples of the various components in the mixture being analyzed
What are the six items that must be determined in order to establish the sequence of a peptide (or protein)? 1) cleave all disulfide bridges and alkylate resulting thiol groups, 2) determine constituent amino acids, 3) identify N-terminal aa, 4) identify C-terminal aa, 5) fragmentation through partial hydrolysis, 6) identify locations of any disulfide bridges
What conditions are employed to "completely" hydrolyze a peptide? Digest peptide in 6 M HCl at 100 degrees C for 24 hours
An enzyme that catalyzes the hydrolysis of a peptide bond at the end of the peptide chain is called? Exopeptidase
Created by: knye



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