| Question | Answer |
| 32. Which of the following microbial forms have the highest resistance to physical and chemical controls?
Naked viruses
Protozoan cysts
Fungal spores
Bacterial endospores
Yeast | Bacterial endospores |
| 33. The process that destroys or removes all microorganisms and microbial forms including bacterial endospores on inanimate objects is ______.
disinfection
sterilization
antisepsis
sanitization
degermation | sterilization |
| 34. The process of using a cleansing technique to mechanically remove and reduce microorganisms and debris to safe levels is _______.
disinfection
sterilization
antisepsis
sanitization
degermation | sanitization |
| 35. The use of a physical or chemical process to destroy vegetative pathogens is _______.
disinfection
sterilization
antisepsis
sanitization
degermation | disinfection |
| The use of chemical agents directly on exposed body surfaces to destroy or inhibit vegetative pathogens is ______.
disinfection
sterilization
antisepsis
sanitization
ionization | antisepsis |
| 2. Scrubbing or immersing the skin in chemicals to reduce the numbers of microbes on the skin is _______.
disinfection
sterilization
antisepsis
sanitization
ionization | antisepsis |
| Which of the following types of control agents would be used to achieve sterility?
Virucide
Bactericide
Germicide
Sporicide
Fungicide | Sporicide |
| 36. The easiest microbial forms to kill or inhibit are ________.
prions
vegetative bacteria and fungi
endospores
protozoan cysts
mycobacteria and staphylococci | vegetative bacteria and fungi |
| 37. The method of removing vegetative microbial life forms from inanimate objects is termed _______.
antisepsis
disinfection
sterilization
decontamination
degerming | disinfection |
| The method of removing vegetative life forms from living surfaces is termed _______.
antisepsis
disinfection
sterilization
decontamination
degerming | antisepsis |
| 38. The removal of all life forms from inanimate objects is termed _______.
antisepsis
disinfection
sterilization
decontamination
degerming | sterilization |
| 4. The betadine swab before blood donation is an example of _______.
antisepsis
disinfection
sterilization
decontamination
sanitation | antisepsis |
| 6. The alcohol wipe before an injection is an example of _______.
antisepsis
disinfection
sterilization
decontamination
virilization | antisepsis |
| A cleansing method that mechanically removes microbes and other debris to reduce contamination is _______.
disinfection
sterilization.
antisepsis
sanitization
degermation | sanitization |
| Which is correct regarding the rate of microbial death?
Cells die at increasingly greater rates.
Only older cells die in a culture.
Cells in a culture die at a constant rate.
Upon contact with the control agent, all cells die at one time.
Cells become metabolically inactive, but are never killed. | Cells in a culture die at a constant rate. |
| 7. Which of the following factors will influence the action of antimicrobial agents?
The number of microorganisms
The kind of microorganisms
Temperature and pH
Mode and dosage of the agent
All of these will influence the action. | All of these will influence the action |
| 39. Microbial death occurs when there is ________.
no movement
permanent loss of reproduction
a change in appearance
a decrease in size
All of these occur | permanent loss of reproduction |
| Each of the following is the target of antimicrobial agents except ______.
cell walls
cell membranes
ribosomes
cellular proteins
cytoplasm | cytoplasm |
| 3. Some microbial control agents are able to _______ cell proteins by breaking bonds that maintain the native state (three-dimensional configuration) of the proteins.
denature
bind
dissolve
activate
All of the choices are correct. | denature |
| 10. Agents that can denature microbial proteins include all of the following except _______.
moist heat
alcohol
acids
metallic ions
X rays | X rays |
| Which of the following does not affect microbial nucleic acids?
Moist heat
Ultraviolet light
X rays
Ethylene dioxide
Formaldehyde | Moist heat |
| 43. Physical agents for controlling microbial growth include all the following except _______.
ultraviolet radiation
boiling water
HEPA filters
pasteurization
hydrogen peroxide | hydrogen peroxide |
| 44. Sterilization is achieved by ________.
flash pasteurization
hot water
boiling water
steam autoclave
All of the choices are correct | steam autoclave |
| 42. Dry heat ________.
is less efficient than moist heat
cannot sterilize
includes tyndallization
is used in devices called autoclaves
will sterilize at 121°C for 15 minutes | is less efficient than moist heat |
| The most efficient sterilizing conditions in a steam autoclave are ________.
121°C at 15 psi for 15 minutes
63°C for 30 minutes
160°C for 2 hours
71.6°C for 15 seconds
100°C for 30 minutes | 121°C at 15 psi for 15 minutes |
| 40. The shortest time required to kill all the microbes in a sample at a specified temperature is called the ________.
thermal death point (TDP)
thermal death time (TDT)
sporicidal time
death phase point
None of the choices are correct | thermal death time (TDT) |
| 41. The lowest temperature needed to kill all microbes in 10 minutes is the ________.
thermal death point (TDP)
thermal death time (TDT)
sporicidal time
death phase point
None of the choices are correct | thermal death point (TDP) |
| 11. Disinfection of beverages, such as apple juice, milk, and wine, is optimally achieved by _______.
pasteurization
chlorination
moist heat autoclave
hydrogen peroxide
boiling water | pasteurization |
| Placing organisms at 4oC is _______.
bactericidal
bacteriostatic
decontamination
sterilization
None of the choices are correct | bacteriostatic |
| Pasteurization ________.
kills all vegetative forms
reduces the number of vegetative forms
reduces the number of endospores
increases food nutrient value
None of the choices are correct | reduces the number of vegetative forms |
| 45. ______ heat is more rapidly effective and efficient compared to _______ heat.
High; dry
High; moist
Dry; moist
Moist; dry
Moist; high | Moist; dry |
| What instrument is most effective for pressure-temperature sterilization?
Oven
Autoclave
Water bath
Bunsen burner
Incubator | Autoclave |
| Vials of microorganisms that undergo the freeze-drying process called _______ will remain preserved and viable for years.
desiccation
flash freeze
lyophilization
pasteurization
sterilization | lyophilization |
| Removal of moisture by dehydration is called _______.
desiccation
flash freeze
lyophilization
pasteurization
sterilization | desiccation |
| _______ radiation excites atoms to a higher energy state within molecules such as DNA that then leads to the formation of pyrimidine dimers.
Infrared
Ultraviolet
Gamma
Particle
Ionizing | Ultraviolet |
| 12. Which of the following items are typically irradiated in order to kill microbes?
Meats like ground beef and pork
Human tissues such as heart valves and skin
Operating room air
Surgical gloves
All of the choices are correct. | All of the choices are correct. |
| Which control method would not be a suitable choice for killing Mycobacterium in a capped culture tube?
Ultraviolet (germicidal) light
Gamma rays
121°C at 15 psi for 15 minutes
160°C for 2 hours
All of the choices would kill Mycobacterium in a culture tube. | Ultraviolet (germicidal) light |
| Which of the following forms of radiation is in order from the most penetrating to the least penetrating?
Gamma, cathode, X rays
Gamma, X rays, cathode
Cathode, gamma, X ray
Cathode, X ray, gamma
X ray, gamma, cathode | Gamma, X rays, cathode |
| 13. HEPA filters are used to remove microbes from ________.
air
liquids
human tissues
medical instruments
All of the choices are correct | air |
| 49. _______ is a control method that removes microorganisms rather than inhibiting or killing them.
Boiling
Sterilization
Radiation
Filtration
Disinfection | Filtration |
| 48. Which of the following is not a factor that affects germicidal activity?
The material being treated
The length of exposure
The strength of the germicide
The microorganism being treated
All of these choices are factors. | All of these choices are factors |
| 15. _______ is a halogen used in gaseous and liquid form for large-scale disinfection of drinking water and sewage.
Iodine
Chlorine
Bromine
Fluorine
All of the choices are correct. | Chlorine |
| 16. The compound that is an organic base containing chlorine and two phenolic rings, and is used increasingly for mucous membrane irrigation, obstetrical washes, hand scrubbing, and prepping surgical skin sites is ______.
carbolic acid
chlorhexidine
triclosan.
formalin
quarternary ammonium compounds | chlorhexidine |
| 5. Alcohols ________.
dissolve membrane lipids at concentrations of greater than 50%
can be used for disinfection or antisepsis
are skin degerming agents
are limited in effectiveness due to rapid evaporation
All of the choices are correct | All of the choices are correct |
| 47. The chemical agent that produces highly toxic and reactive free radicals is _______.
cidex
cationic detergents
hydrogen peroxide
chlorhexidine
iodophors | hydrogen peroxide |
| 46. Hydrogen peroxide can be ________.
sporicidal
fungicidal
bactericidal
virucidal
All of the choices are correct | All of the choices are correct |
| All of the following disrupt the cytoplasmic membrane except ________.
detergents
quaternary ammonium compounds
high concentration phenols
iodine | iodine |
| 8. Heavy metals work by ________.
rupturing the cell membrane
inactivating proteins
binding to DNA
dissolving the cell wall
mutating DNA | inactivating proteins |
| 17. Which of the following is not used as an antiseptic?
Iodophor
Chlorhexidine
3% hydrogen peroxide
Betadine (povidone-iodine)
Glutaraldehyde | Glutaraldehyde |
| Ethylene oxide is ________.
sporicidal
only effective with high heat
the active agent in household bleach
used as an antiseptic against anaerobes
a halogen | sporicidal |
| 1. Compare moist heat methods of microbial control to dry heat control? | Moist heat: Steam/boiling water, shorter time to sterility. Methods include boiling, pasteurization, and autoclaving.
Dry heat: Hot air, needs higher temperature and longer time. Methods include incineration and hot air ovens. |
| 9. Surfactants work by ________. | disrupting membrane integrity |
| 14. Discuss all characteristics that pertain to hypochlorites | release hypochlorous acid in solution
cause denaturation of enzymes
used to disinfect water, restaurant, and medical equipment |
| 18. How does Antimicrobial agents can target the cell walls | blocking its synthesis
digesting it
inhibiting peptidogylcan synthesis |
| 19. What mechanism represents the use of osmotic pressure as a microbial control method? | Creating a hypertonic environment, such as salting or sugaring, which draws water out of microbial cells, causing plasmolysis. |
| 20. Which pair of physical control methods will achieve complete sterilization? | Autoclaving and incineration |
| 21. Temperature and time must be considered when determining susceptibility of microbes to lethal conditions. At a specific temperature, the thermal death _____ can be determined, alternatively, the thermal death _____ can be assessed as the lowest temperature that will kill a test organism | thermal death TIME (TDT); thermal death POINT (TDP) |
| 23. Beginning Fall 2017, the FDA banned the use of phenolic compounds, such as triclosan, in consumer soaps and lotions. This is due to ________. | Increased microbial resistance to these chemicals |
| 24. Chlorhexidine kills microbes by ________. | Disrupting cell membranes and denaturing proteins |
| 25. All of the following are disadvantages to using compounds containing heavy metals, except ________. | they may cause allergic reactions in susceptible individuals
organisms can develop resistance to the compounds
they are not effective against endospores
they can be toxic |
| 26. The use of aldehydes as antimicrobial agents is limited due to ________. | their instability when pH or temperature is increased |
| 27. What are the advantages and disadvantages to using ortho-phthalaldehyde and glutaraldehyde | increased cost |
| 28. What are the advantages and disadvantages using ethylene oxide and moist heat sterilization | it can be used to sterilize plastics that would melt in an autoclave |
| 29. A(n) _________ would be used to destroy bacteria on a countertop whereas a(n) _________ would be used on skin prior to making an incision. | disinfectant; antiseptic |
| 30. Choose the microbial control method that neither inhibits nor kills microbes, but instead physically removes them from liquids or air. | Filtration |
| 31. Describe the antimicrobial activity of chlorhexidine. | Targets cell membrane components and denatures proteins |
| 22. Discuss the characteristic of dry heat | it takes up to 4 hours to sterilize instruments in a hot air oven, whereas the Bunsen burner will incinerate an inoculation loop almost immediately
the Bunsen burner can achieve temperatures up to 1,870 degrees C, Whereas the hot-air oven reaches temperatures up to 180 degrees C. |
| Micro Chapter 9 Study Material
Jamie Garrison | |