Busy. Please wait.
or

show password
Forgot Password?

Don't have an account?  Sign up 
or

Username is available taken
show password

why


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.


Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.

Remove ads
Don't know
Know
remaining cards
Save
0:01
To flip the current card, click it or press the Spacebar key.  To move the current card to one of the three colored boxes, click on the box.  You may also press the UP ARROW key to move the card to the "Know" box, the DOWN ARROW key to move the card to the "Don't know" box, or the RIGHT ARROW key to move the card to the Remaining box.  You may also click on the card displayed in any of the three boxes to bring that card back to the center.

Pass complete!

"Know" box contains:
Time elapsed:
Retries:
restart all cards




share
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

Chapter 31 Voet

Transcription

QuestionAnswer
TBIIB bind TBP, recruit Pol II and TFIIF
σ recognize promoter sequence
α recognize promoter sequence (UP element)
β catalytic domain
β' DNA binding domain
σ factors used for the rapid scanning of DNA for promoters
Sigma factor release occurs after transcription initiation
In bacteria, the promoter region is _ bp 5' to splice site 40
Open complex enzyme melts DNA at -10 region
Open complex formation Conformational change in sigma guides the template strand to the active site tunnel
DOes bacteria transcription require a primer in bacteria? No
What bacterial transcription begins with coupling of 2 NTPs
Promoter escape transition between initiation and elongation
Stages of bacterial initiation with aborted transcripts open complex formation and promoter escape
Passive processes of DNA elongation unwinding and re-annealing
Short region at the active site of elongation DNA:RNA hybrid
Rho-independent terminations structure dyad symmetry, string of >6A's in DNA
Rho-independent termination RNA secondary structure (G-C hairpin loop) followed by run of Us. Mechanical stresses causes rU-dA bonds to break and pull ply-U out of active site. DNA: RNA contact is reduced and transcription terminated
Incorporation of these decrease termination I or 5 BrU
Dyad symmetr yin rho depednent terminators caues RNA polymerase to pause, also acts as barrier to nucleses
Rho is this kind of enzyme helicase
Rho can unwind these double helices RNA:DNA, RNA:RNA
How Rho moves Hydrolyze NTP to propel itself along RNA 5 to 3 until it catches RNAP
Rho protein has how many identical subunits 6
Rho is an ATP-_helicase dependent
RNAPI in nuclei, synthesizes precursors to most rRNA. Species-specific promoter recognition
RNAP II In nucleoplasm, sytnhesize mRNA precursors. Depends on transcription factors
TATA (-25) helps determine start site
CCAAT (-70--90) Increases rate of transcription initiation
GGGCGG housekeeping genes, increase rate of initiation
ENhancers work at distance to increase RNAP II
RNAP III nucleoplasm, synthesize precursors of 5S rRNAs, tRNAs, other small nuclear and cystolic RNAs
Organelle polymerase chloroplast and mitochondrial, similar to RNA polymerases
SWI,SNF,NURF proteins Remodel chromatin by mvoing nucleosomes and control transcriptional activity
Intercalating agents- planar aromatic rings that fit in between base pairs (usually GC. They block both DNA and RNA synthesis. Daunomycin, Adriamycin, Actinomycin D, EtBr, Proflavin
Amatoxins poison in mushroom
α-amanitin inhibits Pol II and III
Rifamycin only inhibit prokaryotic polymerases. They bind pol and block elongation from second to third nuc. Does not block initiation or later elongation if added later. This indicates that there is a conformational difference here.
Cordycepin- 3’deoxyadenosine incorporated into growing chain and causes termination. Indicated that elongation is 5’ to 3’.
repressors block RNAP initiation
Activators turn on expression
Attenuators couple transcription and translation
Created by: ScarlettRose