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microlab, ex6
| Question | Answer |
|---|---|
| amylase | enzyme that breaks down starch (carb catabolism) |
| DNAse | enzyme that breaks down DNA (nucleic acid catabolism) |
| Protease | enzyme that breaks down protein (protein catabolism) |
| Lipase | enzyme that breaks down triglycerides (fat catabolism) |
| oxidation-fermentation (O-F) | pathways for glucose catabolism |
| Catalase | breaks down hydrogen peroxide (respiratory catabolism) |
| cytochrome oxidase | electron transport protein (respiratory metabolism) |
| metabolism | biochemical reactions that power the cell by digesting foods |
| catabolic | the part of metabolism that involves breaking down/digesting foods and extracting their chemical energy |
| anabolic reactions | the building up of molecules to make chemicals the cell needs |
| metabolic reactions are performed by _______. | enzymes |
| true or false: you can use enymes to identify certain bacterial species | true |
| true or false: enymes used for digestion produce an acid that is easily detected by a pH indicator in the medium. | true |
| fermentation | fermentation of sugars is an anaerobic process, so fermenters can grow without oxygen (in glucose tubes= fermenters will produce acid in aerobic and anaerobic tubes) |
| facultative anaerobes | don't require oxygen to grow but can grow in the presence of oxygen |
| oxidizers | aerobic bacteria that produce acid from sugars by oxidative pathways; in glucose tube=oxidizer will produce acid in aerobic tube, but not in anaerobic tube |
| non-oxidizers | bacteria that don't produce any acid at all (in glucose tubes=will not produce acid in aerobic nor anaerobic tubes) |
| caseinase | a protease enzyme that breaks down the casein in milk |
| O-F tube inoculation | stab each with a loop that has bacteria, put a few drops of mineral oil in one tube so oxygen can't get in. |
| Catalaze test | put a drop of hydrogen peroxide on a clean SLIDE. poke bacteria with stick and twist stick in the drop of hydrogen peroxide. bubbles mean positive (mark as +). a few bubbles means weak positive (mark as +w). no bubbles means negative (mark as -) |
| catalyze test substrate, enzyme, products | substrate= hydrogen peroxide. enzyme= catalaze. products= water, oxygen |
| cytochrome oxidase test | poke a stick onto bacteria (won't work with broth cultures). press stick into filter paper strip (paper is impregnated with an oxidase agent). dark purple=positive. lavender= negative. no color=negative |
| Amylaze test | [polysaccharide]starch agar plate. if there is a clearing (halo)around the colony, then the bacteria is positive for amylaze |
| amylaze substrate, enzyme, products | substrate=polysaccharide starch. enzyme= amylaze. product= monosaccharide glucose |
| DNAse test (blue plate) | DNA agar plate contains DNA. positive=halo; negative=no halo |
| DNAse substrate, enzyme, product | substrate=nucleic acid DNA. enzyme=DNAse product=nucleotides, deoxyribonucleotides. |
| lipase test | tributyrin (a simple triglyceride) agar plate. hydrolyzes tributyrin into glycerol, fatty acids. obvious halo=positive for lipase. hazy/opaque around colony=negative. |
| lipase substrate, enzyme, product | substrate= tributyrin. enzyme= lipase. product= glycerol, fatty acids. |
| caseinase (protease) test | skim milk agar plate. caseinase hydrolizes casein. halo=positive. no halo=negative. |
| caseinase substrate, enzyme, product | substrate= casein protein in the milk. enzyme= caseinase/protease. products= small peptides and amino acids. |
| O-F tube test | 2 glucose tubes (green) stabbed by loaded loop, on has drops of mineral oil on top. |
| O-F tubes, both yellow (acid) | fermentative[anaerobic] bacteria fermintative can produce acid with or without oxygen present. mark as O-F.(or F) |
| O-F tubes, aerobic tube yellow (acid), anaerobic tube blue/green | aerobic bacteria, oxidized sugar. mark as O. |
| O-F tubes, both still blue/green [aerobic tube may have turned blue at top from alkaline products] | no sugar metabolism, non-oxidizer bacteria. Mark as N-O. |
| O-F tubes, both slightly yellow at top | slow fermentative [slow anaerobic] bacteria, maybe also oxidative [aerobic] bacteria. so slow fermentation of sugar and maybe also oxidation of sugar occurred. mark as O-F. (or F) |
Created by:
cetesauro42