Busy. Please wait.
or

show password
Forgot Password?

Don't have an account?  Sign up 
or

Username is available taken
show password

why


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.


Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.

Remove Ads
Don't know
Know
remaining cards
Save
0:01
To flip the current card, click it or press the Spacebar key.  To move the current card to one of the three colored boxes, click on the box.  You may also press the UP ARROW key to move the card to the "Know" box, the DOWN ARROW key to move the card to the "Don't know" box, or the RIGHT ARROW key to move the card to the Remaining box.  You may also click on the card displayed in any of the three boxes to bring that card back to the center.

Pass complete!

"Know" box contains:
Time elapsed:
Retries:
restart all cards




share
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

MIA

Protein Purification

WooHoo
Gel filtration chromatography (size) big molecules have a shorted path because they cannot fit trough, can only go around. medium is a bit longer. small is longest, can fit through so must go through all.
Dialysis Semi-permeable membrane (excludes material at molecular level), Permeable (allows small molecules and water to pass), Impermeable (does not allow large molecules).
Dialysis for rapid dialysis, maximise area of membrane/volume of solution.
SDS-page Similar to electrophoresis but SDSP added to sample which applies a negative charge to each protein in proportion to its mass. SDS allows proteins to be separated strictly by molecular weight.
Silver stain used to make invisible protein bands visible (BLACK)
Comassie blue makes protein bands blue, less sensitive.
Mass spectrometry Accurate reading of molecular weight, down to a single atom.
Cation-exchange chromatography Has -vely charged polymer beads. Rate of movement determined by net charge at the pH used. More -ve proteins move faster because like charges repel.
Isoelectric focussing IEF Charge on a protein is affected by pH. A pH gradient is set up and an electric current is passed through. When protein reached pI, it stops.
2D-PAGE first dimension = IEF, separation by charge. second dimension = SDS-PAGE, separation by size. decreasing Mr vertically, decreasing pI horizontally.
Created by: nyna