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Biology Quiz 8
Quiz 8 Questions and Answers
| Question | Answer |
|---|---|
| Which of the following is a characteristic of all viruses? | A protein capsid and a nucleic acid genome only |
| The reason for Tag polymerase for PCR is that | it is heat stable and can withstand the temperature changes of the cycler |
| Plasmids are important in biotechnology because the are _______. | a vehicle for the insertion of foreign genes into bacteria |
| Virulent phages undergo a(n) ______ life cycle, whereas temperate phages are cable of undergoing a(n) _______ cycle. | lytic; lysogenic |
| Which of the following is used to make complementary DNA (cDNA) from RNA? | Reverse transcriptase |
| Yeast cells are frequently used as hosts for cloning because _______. | all of the answers are correct; they are easy to grow, they can remove introns from mRNA, and they have plasmids |
| How does bacterial cell protect its own DNA from restriction enzymes? | By adding methyl groups to adenines and cytosines |
| A cell that remains entirely flexible in its developmental possibilities is said to be _______. | Totipotent |
| DNA fragments from a gel are transfered to the nitrocellulose paper during the procedure called Southern blotting. The purpose of transferring the DNA from gel to nitrocellulose paper is to _________. | permanently attach the DNA fragments to a substrate |
| DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting. What is the purpose of transferring the DNA from the gel to the nitrocellulose paper? | To attach the DNA fragments to a permanent substrate |
| Which of these statements about prokaryotes is correct? | They divide by binary fusion, without mitosis or meiosis |
| DNA microarrays have made a huge impact on genomic studies because they | Allow the expression of many or even all of the genes in the genome to be compared at once |
| V. Uses ligase to seal plasmid DNA to nonplasmid DNA | III, II, IV, V, I |
| Which of the following describes the transfer of polypeptide sequences to a membrane to analyze gene expression? | Western blotting |
| Bacteria containing recombinant plasmids are often identified by which process? | exposing the bacteria to an antibiotic that kills cells lacking the plasmid |
| In order to identify a specific restriction fragment using a probe, what must be done? | The fragments must be separated by electrophoresis, the fragments must be treated with heat or chemicals to separate the strands of the double helix, and the probe must be hybridized with the fragment |
| Which of the following is a characteristic of the lytic cycle? | A large number of phages are released at a time |
| What is the function of reverse transcriptase in retroviruses? | It uses viral RNA as a template for DNA synthesis |
| In animals, embryonic stem cells differ from adult stem cells in that ________. | Embryonic stem cells are totipotent and adult stem cells are pluripotent |
| How does a bacterial cell protect its own DNA from restriction enzymes? | by adding methyl groups to adenines and cytosines |
| Which of the following are least associated with the others? | Binary fusion |
| In 1997, Dolly the sheep was cloned. Which of the following processes were used? | Fusion of an adult cell's nucleus with an enucleated sheep egg, followed by incubation in a surrogate |
| Why might a laboratory be using dideoxy nucleotides? | To sequence a DNA fragment |
Created by:
allichandler