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pht 100 102

chapter 10 part 2

native americans were decimated by european disease like small pox
1/4 of mothers died from infected doctors & nurses unsterile hands and instruments during child delivery
in 1918 the flu killed 25 million people world wide
asepsis is the absence of infectuous microorganism
sterilization is the process of killing infectuous microorganism which leads to asepsis
5 sterilization methods are heat, drying, mechanical, gas, and chemical
water boiling for 10 minutes kills viruses, fungi, & many vegitative forms
water boiling for more than 10 minutes kills fungus, spores, & hepatitis
autoclaves sterilize with 270`F & psi pressure
most organism are sterilized in 15 min.
dry heat or direct flames kill all organisms
filteration is mechanical sterilization
mechanical sterilization is used for heat sensitivity materials like culture media, enzymes, vaccine, antibiotic solutions, asepsis, & steralization
gas sterilization is used for materials subject to heat distruction
ethylene oxide is used in gas sterilization
gas sterilization requires special aeration equipment
large institutions & manufacturing facilities use gas sterilization equipment
chemical sterilization isn't 100% effective
in chemical sterilization, microbial numbers are reduced to safety levels
iodine, isopropyle alcohol (ipa), & chlorine bleach are topical disinfectant
air, water, & touching can cause & spread contaminants
hair, skin, & nails carry millions of bacteria
touching is the most common contaminant the easiest to control
tap water, dust particles, & moist dropplets carry microorganisms
Created by: wildap



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