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Biochem 2
| Question | Answer |
|---|---|
| The ratio of enzyme activity relative to total protein is called | Specific Activity |
| The first step in protein purification from a homogenate is usually | Centrifuge |
| A type of purification that is based on the attraction of the protein for a particular chemical group. | Affinity Chromatography |
| can be added prior to gel electrophoresis to denature the proteins. | SDS |
| Sedimentation coefficients are described as ____________ units. | Svedburg |
| Proteins with different sedimentation coefficients can be separated by ____________. | Zonal Centrifugation |
| In order to sequence a whole protein, ____________ are used. | Overlapping |
| The name used to describe the original, uncleaved protein. | Nascent |
| Another name for an antibody. | Immunoglobulin |
| Another name for an antigenic determinant. | Epitope |
| Proteins can be separated from small molecules and ions through a semi-permeable membrane by | Dialysis |
| Exclusion gel or gel-filtration chromatography separates molecules on the basis of | Size |
| __________________ is a chemical reagent that is often used to detect the presence of amino acids. | ninhydrin |
| In the Edman procedure for peptide sequence, phenyl isothiocyanate is used to selectively remove the __________________ residue as a PTH-derivative. Section | N-terminal |
| Disulfide bonds in peptides and proteins are readily oxidized to cysteic acid residues by treatment with | Performic acid |
| MALDI-TOF is the abbreviation for __________________. | Matrix Assisted Laser Desorption Ionization-Time of Flight |
| Automated peptide synthesis involves the activation of the carboxyl group of the incoming amino acid by __________________ and then reaction with the amino group of the growing peptide chain. | DCC |
| Polypeptides can be fragmented into smaller peptides by cleavage with chymotrypsin, which hydrolyzes the peptide bond at the C-terminal side of __________________ | Leucine, methionine, tryptophane, tyrosine, and phenylalanine |
| __________________ gels are often used as the media for electrophoretic techniques such as SDS-PAGE and isoelectric focusing | Polyacrylamide |
| The mobility of proteins in SDS-PAGE is inversely proportional to the _____________. | Mass |
| When enzymes are purified, the assay is often based on | catalytic activity |
| Proteins that are not catalysts are often assayed using | antibody binding assays |
| What is the advantage of adding SDS to gel electrophoresis? | SDS allows proteins to be separated on the basis of approximate mass. |
| Two-dimensional electrophoresis is a combination of what two techniques? | isoelectric focusing and SDS-PAGE |
| Which of the following affect the sedimentation of a particle? | mass, shape, and density |
| Cyanogen bromide cleaves the peptide bond at | the carboxyl side of Met residues. |
| Trypsin cleaves the peptide bond at | the carboxyl side of Arg and Lys residues. |
| Which of the following techniques can be used to determine the site of a disulfide bond? | diagonal electrophoresis |
| What types of molecules can serve as antigens? | proteins and polysaccharides |
| An ELISA can be used for | quantitative analysis. |
| A technique used to identify proteins after gel electrophoresis, which employs antibodies in the detection process. | Western Blot |
| A technique that can be used to study protein location in cells. | fluorescent microscopy |
| The use of synthetic peptides includes | use as antigens for making antibodies, drugs, hook used in purification. |
| Which technique cannot be used for quantitative analysis? | X-ray crystallography |
| Techniques that can be used to obtain information about protein shape are | X-ray crystallography and NMR spectroscopy. |
| The information-carrying intermediates of translation. | M-RNA |
| The flow of genetic information from DNA to protein is called ___________ | Gene expression |
| Scientist who noted the A:T and G:C ratios in DNA are approximately | Erwin Chargaff |
| The temperature at which half the DNA helical character is lost is referred to as ____________. | Tm |
| DNA renaturation after melting is called ____________. | Annealing, |
| DNA can exist in both circular and ____________ forms. | Linear |
| Codons that specify the same amino acids are termed _______ | Synonyms |
| A three-base codon defines a specific ____ | Amino acid |
| Stop codons are read by ____ | Release factors |
| The enzyme that is capable of synthesizing DNA from an RNA template is ___________ | Reverse transcriptase |
| The nucleoside that is composed of a D-ribose linked to a cytosine base is called ___________ | Cytidine |
| The phenomenon that stacked bases in double-helical DNA absorbed less UV light than the unstacked bases in single-stranded DNA is referred to as _____ | hypochromicity |
| The difference in RNA bases compared to DNA bases is | RNA contains U instead of T. |
| How does a nucleotide differ from a nucleoside? | A nucleotide is a nucleoside with a phosphate ester linked to the sugar |
| What is the nucleotide sequence on the DNA template strand that yields the CUA codon for leucine? | TAG |
| How many different sequence possibilities are there for a DNA polymer that is 10 bases long? | 1,048,576 (4^10) |
| The technique(s) used by Franklin and Wilkins to deduce the structure of DNA was | X-ray diffraction. |
| The feature(s) of DNA deduced by Watson and Crick included | two antiparallel polynucleotide chains coiled in a helix around a common axis. the pyrimidine and purine bases lie on the inside of the helix. the bases are nearly perpendicular to the axis. |
| The chemical forces that contribute to the stability of the DNA due to the base stacking present in the DNA helix are | van der Waals |
Created by:
farfar08