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Lab #1
Brightfield Microscopy
Questio | Answer |
---|---|
A microscope that allows light rays to pass directly to the eye without being deflected by an intervening opaque plate in the condenser. | Brightfield microscopy |
How should you transport your microscope? | When carrying your microscope from one part of the room to another, use both hands to hold the instrument. |
All microscopes have a basic frame structure, which includes the ___ and ___. | Arm; base. |
The horizontal platform that supports the microscope slide is called the ___. | Stage. |
The clamping device is called ___, which is used for holding and moving the slide around the stage. | Stage adjustment. |
The knurled wheel on the right side of the base to regulate the voltage supplied to the lightbulb is called ___ ___ ___. | Light intensity control. |
The ___ ___ ___ is often needed to reduce the intensity of light below the lower limit allowed by the voltage control. You can place it over the light source in the base, or in some microscopes, it is built into the base. | Neutral density filter. |
All compound microscopes have three lens systems: ___ ___ ___. | Oculars, objectives, condenser. |
This complex piece is located at the top of the instrument, which consists of two or more internal bases and has a magnification of 10x. Most have two lenses. | Ocular (eyepiece). |
Three or more ___ are present, attached to a rotatable ___, which makes it possible to move them into position over a slide. | Objectives. |
Objectives have magnifications of: __, __, __. | 10x (low-power), 40x (high-dry), 100x (oil immersion). |
How do you calculate the total magnification? | Multiply the power of the ocular lens (10x) by the power of the objective lens used (10x or 40x, or 100x). |
Which third lens system is located under the stage? It collects and directs the light from the lamp to the slide being studied. It does not affect the magnifying power of the compound microscope. It can be moved up and down by a knob under the stage. | Condenser. |
A ___ within the condenser regulates the amount of light that reaches the slide. | Diaphragm. |
The conce |