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Lab Safety
Biology Lab Safety study questions
| Question | Answer |
|---|---|
| Before performing an experiment______ahead of time. | read through it |
| Never perform a(n)______experiment. | unauthorized |
| Lab is not the place for_______. | fooling around |
| Do not______. | "cut corners" |
| Three basic parts to lab fashion include: | lab coats, gloves, and safety goggles |
| Three things that should be secured before starting a lab: | hair, loose clothes, and jewelry |
| If you have extra chemicals, never______. | put them back in the bottle |
| When performing combining chemicals, always follow steps_______. | in order/exactly |
| What is MSDS? | Material Safety Data Sheet |
| Always read labels and work with chemicals from ______ bottles. | labeled |
| When diluting acids follow the ______ rule which is _______. | Always Add Acid (to water, never the other way around) |
| When working with living materials, ______________the bench with_______and then______. | wipe down; water; ethanol |
| Two pieces of equipment used for sterilizing are: | bunsen burner and autoclave |
| Use two pairs of gloves when working with _______. | very poisonous materials |
| To avoid contamination you should _______ gloves when appropriate and_______ gloves before leaving the lab. | change; remove |
| When heating a test tube, _______ the tube slightly away and the end of the tube should be _______ the flame. | point/tilt; above |
| Every accident, no matter how small should be _______. | reported to the teacher |
| Do not use glass that is_______. | cracked, chipped, or broken |
| Bare hands should not be used for cleaning up _______ glass. | broken |
| Clean up after a lab should include wiping down ______ and _______ and ______ lab equipment properly. | tables; test tubes; store |
| Never eat, drink, or _______ in a lab. | chew gum |
| How should you conduct yourself in a laboratory? | in a responsible manner at all times |
| What should you follow very carefully? | all written and verbal instructions; if you do not understand a direction or part of a procedure, ask the instructor before proceding |
| Never work ______. | alone; no student may work in the laboratory without an instructor present. |
| When first entering a science room, _______. | do not touch any equipment, chemicals, or other materials in the thaboratory area until you are instructed to do so. |
| Do not eat _______. | food, drink beverages, or chew gum in the laboratory. Do not use laboratory glassware as containers for food or beverages. |
| Perform only those experiments that are _______. | authorized by the instructor. Never do anything in the laboratory that is not called for in the laboratory procedures or by your instructor. |
| What should you do before entering the laboratory? | be prepared for your work; read all procedures thoroughly; never fool around; horseplay, practical jokes, and pranks are dangerous and prohibited. |
| Work areas should be _______. | kept clean and tidy at all times; bring only your laboratory instructions, worksheets, and/or reportes to the work area. Other materials should be stored in the classroom area. |
| Keep _______ clear. | aisles. push your chair under the desk when not in use |
| Know the locations and operating procedures of _______. | all safety equipment, including the first aid kit, eyewash station, safety shower, fire extinguisher, and fire blanket. Know where the fire alarm and the exits are located |
| Always work in a _______ area. | well-ventilated; use the fume hood when working with volatile substances or poisonous vapors. never place your head into the fume hood. |
| Be _______ and procede with _______ at all times in the laboratory. | alert; caution; noify the instructor immediately of any unsafe conditions you observe. |
| Dispose of _______ properly. | all chemical waste; never mix chemicals in sink drains; sinks are to be used only for water and those solutions designated by the instructor |
| Read _______ carefully before use. | Labels and equipment instructions; set up and use the prescribed apparatus as directed in the laboratory instructions or by your instructor. |
| Keep hands _______. | away from face, eyes mouth, and body while using chemicals or preserved speciments. Wash your hands with soap and water after performing all experiments |
| Experiments must be _______ at all times | personally monitored; you will be assigned a laboratory station at which to work; do not wander around the room, distract other students, or interfere with the laboratory experiments of others. |
| Students are never permitted in _______. | the science storage rooms or preparation areas unless given specific permission by their instructor. |
| What do you do if there is a fire drill during a laboratory period? | all containers must be closed, and any electrical equipment turned off. |
| Handle all living organisms used in a laboratory activity in a _______ . | humane manner; preserved biological materials are to be treated with respect and disposed of properly |
| When using knoves and other sharp instruments, _______. | always carry with tips and points pointing down and away. Always cut away from your body; never try to catch falling sharp instruments; grasp sharp instruments only by the handles |
| Wear laboratory goggles any time _______. | chemicals heat or glasware are used; no exeptions! |
| _______ should not be worn in the laboratory unless you have permission from your instructor | contact lenses (unless otherwise indicated, contacts are OK for Biology Labs) |
| _______ are have been provided for your use and should be worn during lab activities | lab aprons |
| What should you do after every accident or injury,no matter how small? | report it to the teacher |
| What should you yell immediately if either you or your partner are hurt? | "Code One! Code One!" |
| What should you do if a chemical splashes in your eyes or on your skin? | immediately flush with running water from the eyewash station or safety shower for at least twenty minutes; notify the instructor immediately |
| When mercury thermometers are broken, _______. | mercury should not be touched; notify the instructor immediately |
| All chemicals in the lab are to be considered _______. | dangerous; do not touch, taste, or smell any chemicals unless specifically instructed to do so. |
| Check the _______ on chemical bottles _______ before remoing any of the contents. | label; twice; take only as much chemical as you need |
| Never return _______. | used chemicals to their original containers |
| Never use mouth suction to _______. | fill a pipet; use a rubber bulb or pipet pump |
| wWhen transferring reagents from one container to another, _______ | hold the containers away from your body |
| _______ must be handled with extreme care; | acid; always add acid to water, swirl or stir the solution and be careful of the heat produced, particularly with sulfuric acid |
| Take great care when transferring _______. | acids and other chemicals from one part of the laboratory to another; hold them securely and walk carefully |
| Carry glass tubing, especially long pieces, _______. | in a vertical position to minimize the likelihood of breakage and injury; never use chipped or broken glassware |
| Never handle broken glass with _______. | your bare hands; use a brush and dustpan to clean up broken glass; place broken waste glassware in the designated glass disposal container |
| Always _______ glassware before attempting to insert it in a stopper. Always protect your hands with _______. If a piece of glassware becomes "Frozen" in a stopper, _______. | lubricate; towels or cotton gloves when inserting glass tubing into, or removing it from, a rubber stopper; take it to your instructor for removal |
| When removing an electrical plug from its socket, grasp _______. Hands must be _______ before touching an electrical switch, plug, or outlet. | the plug, not the electrical cord;; completely dry |
| Examine _______ before each use. | glassware; Never use chipped or cracked glassware; never use dirty glassware |
| Report damaged electrical equipment _______. | immediately; look for things such as frayed cords, exposed wires, and loose connections; do not use damaged electrical equipment |
| If you do not understand how to use a piece of equipment, ______. | ask the instructor for help |
| Do not immerse hot glassware _______. | in cold water; it may shatter |
| Always carry microscopes _______. | with two hands; remove slides from the stage before returning the microscope to the storage area |
| Never place a microscope _______. | at the edge of a lab table |
| Exercise extreme caution when using ______. Make sure that ________ are a safe distance away at all times. Never reach over _______. | gas burner; hair, clothing, and hands; an open flame |
| Do not point the open end of a test tube being heated | at yourself or anyone else. |
| ________ remain hot for a very long time. | Heated metals and glass; they should be set aside to cool and picked up with caution; use tongs or heat-protective gloves if necessary |
| Never look into | a container that is being heated |
| Do not place hot apparatus | directly on the laboratory desk; always use an insulating pad; allow plenty of time for hot apparatus to cool before touching it |
| Always put _______ away as directed by your instructor. | materials and equipment; clean off your lab table with a damp paper towel if necessary; return supplies to the supply table and make usre the supply table is in order and clean |
| Materials and supplies should be used | in a manner that respects Earth's limited resources |
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delucao15