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Serous Fluid
Urinalysis and Bodily Fluids-Serous Fluid
| Question | Answer |
|---|---|
| What is serous fluid? | Fluid between the inner & outer layers of serous membranes (parietal & visceral) lining closed cavities of the body |
| What is the function of serous fluid? | REDUCES FRICTION (LUBRICATION) |
| What are the 3 types of serous fluids and where are they from? | 1.PLEURAL MEMBRANE SURROUNDING LUNGS 2.PERICARDIAL MEMBRANE AROUND HEART 3.PERITONEAL (ASCITES) MEMBRANE AROUND ABDOMINAL ORGANS |
| What are the 2 ways that serous fluid is formed? | 1.ULTRAFILTRATE OF PLASMA 2.EFFUSIONS INCREASED FLUID THAT FORMS WHEN DISRUPTION OF THE BALANCE BETWEEN FORMATION & REABSORPTION OCCURS |
| Which formation of serous fluid is abnormal? | Effusions |
| How is serous fluid collected and how much ? | BY NEEDLE ASPIRATION COLLECT 100 ml |
| Where is the serous fluid distributed and what kind of preservatives for each? | DISTRIBUTED INTO 2 TUBES LIQUID EDTA FOR HEMATOLOGY HEPARINIZED FOR OTHER TESTS |
| How soon is Serous fluid tested after capture? | PERFORM TESTING ASAP |
| How are SPECIMENS FOR pH DRAWN (FOR SEROUS)? | ANAEROBICALLY ON ICE |
| What is THORACENTES(FOR SEROUS)? | PUNCTURE OF CHEST PLEURAL FLUID |
| What is PERICARDIOCENTESIS (FOR SEROUS)? | PUNCTURE OF PERICARDIUM PERICARDIAL FLUID |
| What is PARACENTESIS(FOR SEROUS)? | PUNCTURE OF ABDOMINAL CAVITY PERITONEUM ASCITES (PERITONEAL FLUID) |
| Transudates (FOR SEROUS)? | FLUID FORMED WHEN BALANCE BETWEEN FLUID FILTRATION & REABSORPTION IS DISRUPTED NOTHING WRONG WITH MEMBRANE, BUT SUBSTANCES ARE MOVING ACROSS MEMBRANE INTO TRANSUDATE |
| What 2 illnesses are associated with transudates(FOR SEROUS)? | CHF; NEPHROTIC SYNDROME |
| Exudates (FOR SEROUS)? | Fluid formed due to conditions directly involving the membranes themselves. |
| What 2 types of problems are assoc. with exudates(FOR SEROUS)? | infections and malignancies |
| Exudate or Transudate (FOR SEROUS)? | CLEAR <1000 WBCs/ul < 60 mg/dl CHOL <0.5 (fluid:serum protein ratio) No spontaneous clotting |
| Exudate or Transudate(FOR SEROUS)? | CLOUDY >1000 WBCs/ul RBC > 100,000/ul > 60 mg/dl CHOL > 0.5 (fluid:serum protein ratio) Spontaneous clotting possible |
| For a virus, what wbcs will be seen in a diff (FOR SEROUS)? | lymphs and monos |
| For a bacteria, what wbcs will be seen(FOR SEROUS)? | segs |
| (FOR SEROUS) what 2 types of cells are counted? | wbcs and rbcs |
| (FOR SEROUS) what stain is used for a cytocentrifuged sample? | Wright Stain |
| What types of cells are looked for on a diff (FOR SEROUS)? | wbc, normal and malignant tissue cells |
| What for chemistry tests are performed (FOR SEROUS)? | glucose, pH, amylase, and triglycerides |
| For Pleural Fluid what 2 types? | transudate or exudate |
| For Pleural Fluid what is the normal appearance and viscosity? | transudate is normal as clear and pale yellow |
| For Pleural Fluid what does the abnormal exudate appear as (3 possibilities and explanations) | 1.turbid-wbcs & infection/inflammation 2.bloody-hemothorax 3.milky-chylous (fatty)-trig present |
| For Pleural Fluid give the cholesterol levels for transudate v. exudate: | trans-chol<60 mg/dL exudate-chol>60mg/dL |
| For Pleural Fluid give the fluid to serum chol ratios for trans v. exudate: | trans-fluid to serum chol ratio=<0.3 exudate-fluid to serum chol ratio=>0.3 |
| For Pleural Fluid under what # should wbcs fall for a transudate? | <1000/ul |
| For Pleural Fluid what cells are normal for transudate? | some neutrophils, mostly lymphs, eos and mesothelial cells |
| For Pleural Fluid Hematology tests, increased neutrophils would indicate? | infection (pneumonia) |
| For Pleural Fluid Hematology tests, increased lymphs would indicate what 4 possibilities? | tb, viral, malignancy, Lupus (SLE) |
| For Pleural Fluid Hematology tests, increased eosinophils would indicate what 3? | trauma, allergy or parasites |
| For Pleural Fluid Hematology tests, no reactive mesothelial cells would indicate? | TB |
| For Pleural Fluid Hematology tests, LE cells would indicate? | autoimmune disorder SLE |
| For Pleural Fluid Hematology tests, describe mesothelial cells | single, small or large, round cells with abundant blue cytoplasm and round nucleii with uniform dark purple cytoplasm |
| For Pleural Fluid chemical tests, what would decreased glucose (<60 mg/dL) be assoc. with (2)? | 1.RA 2.inflammation or purulent infection |
| For Pleural Fluid chemical tests, what does increased Lactate indicate? | bacterial infections |
| For Pleural Fluid chemical tests, a pH of <7.3 indicates? | need chest tube drainage |
| For Pleural Fluid chemical tests, a pH of <6 indicates? | esophageal rupture |
| For Pleural Fluid chemical tests,when is amylase increased? | pancreatitis |
| For Pleural Fluid chemical tests,what does triglyceride confirm? | chylous effusions |
| For Pleural Fluid mircobiology tests,what 2 tests and stains are performed? | 1.culture and gram stain 2.TB culture and AFB stain |
| For Pleural Fluid serology tests,what 3 tests are performed? | 1.ANA 2.RF 3.CEA Tumor marker (carcino embryonic antigen) |
| For PERICARDIAL Fluid what is the normal appearance and viscosity? | normal and transudate are clear and pale yellow |
| For PERICARDIAL Fluid what are the abnormal/effusions the same as? | Pleural Fluid except the bloody would be an accidental cardiac puncture |
| For PERICARDIAL Fluid, what would be indicative of bacterial endocarditis? | >1000/uL wbcs with high % neutrophils |
| For PERICARDIAL Fluid, what would the presence of malignant cells indicate? | metastatic lung or breast cancer |
| For PERICARDIAL Fluid what tests in microbiology would be performed for bacterial endocarditis? | culture and gram stain |
| For PERITONEAL FLUID (ASCITES), what is the normal appearance and viscosity? | clear and pale yellow |
| For PERITONEAL FLUID (ASCITES) what would turbidity suggest? | wbcs & infection/inflammation |
| For PERITONEAL FLUID (ASCITES) what would green (bile) suggest? 2 | gallbladder, pancreas |
| For PERITONEAL FLUID (ASCITES) what would bloody suggest? 3 | trauma, infection, malignancy |
| For PERITONEAL FLUID (ASCITES) what would milky suggest? | chylous (fatty) =trauma or lymph blockage |
| For PERITONEAL FLUID (ASCITES) what is the normal wbc count with how many neutrophils? | <500 with <50% neutrophils |
| For PERITONEAL FLUID (ASCITES) under 50% neutrophils and greater than 500 wbcs indicates | Cirrhosis |
| For PERITONEAL FLUID (ASCITES) what do malignant cells contain? what does this indicate? | mucin filled vacuoles which indicates metastatic tumors |
| For PERITONEAL FLUID (ASCITES) what does decreased glucose indicate? 2 | 1.TB peritonitis 2.malignancy |
| For PERITONEAL FLUID (ASCITES) when is amylase increased? | pancreatitis |
| For PERITONEAL FLUID (ASCITES) what does elevated alkaline phosphatase indicate? | intestinal perforations |
| For PERITONEAL FLUID (ASCITES) what is a positive BUN and Creatinine indicative of? | ruptured bladder |
| For PERITONEAL FLUID (ASCITES) what is being tested for in a culture and gram stain? | bacterial peritonitis |