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chromatography
| Question | Answer |
|---|---|
| What is the Rf value? | The retention factor. This is calculated by distance travelled by spot divided by the distance travelled by the solvent front. |
| What is the process by which a solid holds a molecule of gas or liquid? | Adsorption |
| What is the mobile phase? | The phase that moves |
| What is the stationary phase? | The phase that does not move |
| What basis does chromatography work on? | It works on the basis that different components have different affinities for a stationary and for a mobile phase. |
| In thin layer chromatography what is the stationary and mobile phase? | The stationary phase is a solid and the mobile phase is a liquid. |
| In gas chromatography what is the stationary and mobile phase? | The stationary phase is liquid or solid on a solid support and the mobile phase is a gas. |
| What are the two main factor efficient separation depends on? | A solid stationary phase that separates by adsorption and a liquid staationary phase that separates by relative solubility. |
| What is retention time? | The time for a component to pass from the column inlet to the detector. |
| On a chromatagram what is the area under each peak proportional to? | The amount of a compound in the sample. |
Created by:
sophs