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chromatography

QuestionAnswer
What is the Rf value? The retention factor. This is calculated by distance travelled by spot divided by the distance travelled by the solvent front.
What is the process by which a solid holds a molecule of gas or liquid? Adsorption
What is the mobile phase? The phase that moves
What is the stationary phase? The phase that does not move
What basis does chromatography work on? It works on the basis that different components have different affinities for a stationary and for a mobile phase.
In thin layer chromatography what is the stationary and mobile phase? The stationary phase is a solid and the mobile phase is a liquid.
In gas chromatography what is the stationary and mobile phase? The stationary phase is liquid or solid on a solid support and the mobile phase is a gas.
What are the two main factor efficient separation depends on? A solid stationary phase that separates by adsorption and a liquid staationary phase that separates by relative solubility.
What is retention time? The time for a component to pass from the column inlet to the detector.
On a chromatagram what is the area under each peak proportional to? The amount of a compound in the sample.
Created by: sophs