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Sperm collection

QuestionAnswer
How is bull semen collected? An artificial vagina
What are the parts of a bovine artificial vagina? A warm water jacket and a semen vial
Explain the process of collection for an artifcial vagina Allow the bull to mount another bull and slip the artificial vagina over the penis for ejaculation
What is the tube of semen evaluated for? Volume, concentration, total sperm per ejaculate, progressive motility, morphology
Desired range for ejaculation volume 5 (1-15) mL
Desired range for sperm concentration 1200 million (300-2500 million)
Desired total sperm per ejaculate 4-5 billion
Desired progressive motility Greater than 30%
What does progressive motility mean? The sperm cells are moving forward in a straight line and not swimming in circles
Desired morphology Greater than 70% normal
What does morphology look for? Whether the sperm is dead or alive and for abnormalities
Evaluations completed under a microscope Progressive motility and morphology
How is sperm diluted? By being put in a flask and adding a dilutant
Components added to frozen semen Salt, buffer, glycerol, glucose, extenders, antibiotics
Extenders for frozen semen Milk or egg yolk to protect during cooling
Purpose of glycerol in frozen semen Protect sperm during freeze
Purpose of glucose in frozen semen Energy source
Purpose of antibiotics in frozen semen Prevent bacterial growth
Why is sperm put in liquid nitrogen tanks? It can be stored in there for a very long time and shipped all across the world
Sire Conception Rate (SCR) Collected from worldwide data to show if the sire's sperm is actually getting cows pregnant
What happens if you have a negative SCR? Cull the sire
Biggest sex-sorted semen company Sexin Technologies (ST)
Advantages of sex-sorting semen Ensures there will be mostly replacement heifers (90%), cows you don't want to generate a replacement can be given beef semen
Disadvantages of sex-sorting semen More expensive, lower fertility rates
Karyotype A picture of all the chromosomes in the body
Why does sperm sorting work? X bearing sperm has more DNA than Y bearing sperm
X bearing sperm A sperm with an X chromosome that will produce a female calf
Y bearing sperm A sperm with a Y chromosome that will produce a male calf
Difference between stains in sexed sperm X bearing sperm will stain darker than Y bearing sperm because it has more DNA
How is sperm sorted? A flow cytometer
How does a flow cytometer sort sperm? Using a laser to detect the stain and sort based on that
How does sex-sorting semen affect the straws? You get less straws per ejaculate. 150
What does Ultra 4M mean? You need 4 million sperm cells in a straw
Created by: user-2052955
 

 



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