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Lab Practical Exam 1

Microbiology Lab Paractical Exam # 1

QuestionAnswer
Quiz #1 1. Contamination Lab: The BHI, NA and SDA plates all had the same nutrients. False
Quiz #1 2. Microscope lab: The objective lens magnification is 10X and the ocular lens magnification is 10X. What is the TOTAL magnification? 100X
Quiz #1 3. Contamination Lab: Two students swabbed the same desk using cotton swabs. Student A spread the swab onto a nutrient agar (NA) plate and incubated it at 20°C. Student B also spread the swab onto a nutrient agar (NA) plate, but incubated it at 3 Because the optimal temperature of the incubation chamber has a different ratio student A has incubated NA plate of 20'C recive no reaction. Student B NA plate incubator ratio of 35'C bacterial growth is present.
Quiz #1 4. Contamination Lab: What are the possible sources of contamination for this lab? Correct answer: all of the above Air bare fingers gloved fingers "Correct answer: all of the above "
Quiz #1 5. Contamination Lab: you will be able to identify the exact type of bacteria you have growing on your plate. False
Quiz #2 1. The glass slide and __________ have approximately the same refractive index, which helps reduce the refraction (bending) of light Oil
Quiz #2 2. During the STD lab, we looked at 4 different microorganisms. Which microorganism is a prokaryote? Candida albicans
Quiz #2 3. For the Live Protozoa lab, we are looking at 3 protozoa. Select the structures that would help with motility in these protozoa. Correct answer: all of the above flagella cilia pseudopods
Quiz #2 4. What shape is this bacteria seen in this image? Hint : Red spiralla shape bacteria spiral
Quiz #2 5. The protozoa (Euglena, Paramecium and Amoeba) are all eukaryotes True
Quiz # 3 1. Before staining, what did we use to fix the microorganism on the slide? Heat
Quiz # 3 2. Methylene blue has a _________________ charge and the bacterial cell wall has a ____________ charge. positive charge , negative charge
Quiz # 3 3.Based off this image, you can identify bacteria as: Hint Methlene blue color , rod shape bacteria'sthis could be both B. subtilus or E. coli
Quiz # 3 4. What is the final goal of the streaking technique To isolate the individual colonies in the sample
Quiz # 3 5. What can make disinfectants less effective?A student selected an isolated red bacterial colony from a plate and used it to inoculate (streak) a new plate. Correct answer: all of the above Biofilms Endospores The presence of organic matter in blood, vomit, or feces
Quiz #4 1. Theoretically, which bacteria should be more resistant to the disinfectants used in lab? B. subtilus
Quiz #4 2. A student observed no bacterial growth on the control quadrant. After spraying Sanisol, she observed bacterial growth after 30 seconds, but no bacterial growth after 3 minutes or 10 minutes. What can be concluded from these results? The results are inconclusive.
Quiz #4 3. A student selected an isolated red bacterial colony from a plate and used it to inoculate (streak) a new plate. During the next lab period, she observed both red and yellow bacterial colonies growing on the new plate. What is the most likely e The student did not obtain a pure culture when selecting the isolated colony
Quiz #4 4.What bacteria cell structure does the Gram stain tell us about? cell wall
Quiz #4 5. When observing a Gram-stained slide under the microscope, you see pink, bacilli bacterial cells. Which bacterium are you most likely observing? Escherichia coli
Quiz #4 6. I have been reading the protocol before coming to lab... True
Quiz # 5 1. What is meant by the Minimal Inhibitory Concentration (MIC)? The lowest concentration of an antimicrobial agent that prevents visible bacterial growth.
Quiz # 5 2.A bacteria that shows no inhibition in growth is resistant to the antibiotic True
Quiz # 5 3.Based off what you know about penicillin, which bacteria is going to be more resistant to this antibiotic? E. coli (Gram Negative)
Quiz # 5 4.Antibiotics can treat viral infection such as the cold or the flu. False
Quiz # 5 5. A bacterial culture is tested with increasing concentrations of an antibiotic. The results are shown below: Antibiotic concentration Bacterial growth 1 µg/mL Growth 2 µg/mL Growth 4 µg/mL No growth 8 µg/mL No growth What is the MIC 4 µg/mL
Created by: Rodney C
 



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