click below
click below
Normal Size Small Size show me how
Bio Lab 4
| Question | Answer |
|---|---|
| What four structures do ALL cells (eukaryotes AND prokaryotes) possess? | Plasma/cellular membrane, cytosol/cytoplasm, genetic material (DNA), and Ribosomes |
| What are the major differences between eurkaryotes and prokaryotes? | Their size (pro is bigger), Pro DNA does not contain nucleus/moves freely in cell, Eukaryotic contain nucleus/stays in membrane, cell structures, pro represents monera kingdom/eukaryotic represents plants, animals, fungi, etc. |
| Cell Wall | maintains shape and structure, prevents bursting; in plants |
| Cell Membrane/Plasma | controls what enters/exits the cell; in plants and animals |
| Nucleus | contains DNA; maintains genes and controls activities of the cell; in plants and animals |
| Nucleolus | produces ribosomes; in plants and animals |
| Ribosome | produce proteins; in plants and animals |
| Rough Endoplasmic Reticulum | processes and packages proteins, contain ribosomes; in plants and animals |
| Smooth Endoplasmic Reticulum | No ribosomes; produces and processes lipids, in plants and animals |
| Golgi Apparatus | Sorts and ships organic molecules, in plants and animals |
| Mitochondria | convert organic materials to energy (ATP), in plants and animals |
| Lysosome | contains digestive enzymes to break down organic molecules, in animals |
| Fat Vacuole | storage of fat, in animals |
| Chloroplast | site of photosynthesis, in plants |
| Central Vacuole | Aid in cell division, In plants and animals |
| Cytoplasm | Liquid component containing the organelles, in plants and animals |
| Gram Stain | positive: dark purple/blue negative: red |
| Cell Morphology | Coccus=round shape, Bacillus=rod shape, Spirillum=spiral shape |
| Practice Cell Structure Interactive Map | |
| Magnification | the amount an image is enlarged |
| Resolution=Resolving Power | the extent to which image detail is preserved |
| Contrast | the ability of an image to stand out against its background |
| Working Distance | the vertical space between the front edge of the objective lens and the top surface of the coverslip or focused specimen |
| Field of view = Diameter | multiply diameter (mm) by 1000 to get field of view (micrometers) ; The total circular or rectangular area of the specimen that you can see through the eyepiece or camera at one time. |
| Parfocal | when you change lenses, image will always remain in focus |
| Parcentral | when you change lenses, image will always stay in center of your field of view |
| Light Microscopes Characteristics: | color images, living/non-living, 40x-1000x magnification, compound light and stereo dissecting |
| Light Microscope Types/uses | Compound Light Microscope 2D, uses two lenses at a time, light goes up through specimen Stereo Dissecting Microscope 3D, light shines down on specimen to view outer surface detail |
| Electron Microscopes | black/white images, non-living, 350000x magnification, creates electromagnetic field for magnification, Scanning Electron Microscope , 3D, outer images Transmission Electron Microscope 2D, internal structure |
| Scanning Power | 4x, total magnification: 40x (red), shows the whole slide |
| Low Power | 10x, total magnification: 100x (yellow), shows the object in greater detail |
| High Dry Power | 40x, total magnification: 400x (blue), shows the object in even greater detail |
| Coarse Adjustment Knob | Locates specimen, brings into approximate focus |
| Fine Adjustment Knob | Brings specimen into final focus |
| Arm | Supports upper parts of microscope and serves as a carrying handle |
| Base | Bottom part of microscope, supports stage and arm |
| Body | Connects nosepiece and eyepiece |
| Diaphragm | Regulates the amount of light used to view the object |
| Eyepiece = Oculars | series of lenses(top) through which an object is viewed , magnification is 10x |
| Light source=illuminator | Lamp that directs a beam of light through the specimen |
| Revolving Nose Piece | Revolving piece that holds objectives, lenses used to magnify specimen |
| Scanning Power Objective | 4x, total magnification: 40x (red), shows the whole slide |
| Low Power Objective | 10x, total magnification: 100x (yellow), shows the object in greater detail |
| High dry power Objective | 40x, total magnification: 400x (blue), shows the object in even greater detail |
| Stage | Holds and supports microscope slides |
| Slide Position Adjustment Knobs | Moves slides left to right and front to back |
| Stage Clips | Lever that opens to secure the slide on the stage |
| Label microscope image | |
| Length | The measurement of a line from one point to another Meter (m), Metric Ruler |
| Volume | Amount of space an object occupies Liter (L), Erlenmeyer flask, beaker, graduated cylinder, pipet |
| Mass | Amount of matter in an object Grams (g), Taring (setting scale to zero) |
| Temperature | Degree of how hot or cold an object is Degrees C, Thermometer |
| Meniscus | The curve of the liquid in the container (like a graduated cylinder). |
| Kilo | 1 m = 1000 km |
| Deci | 1 m = 10 cm |
| Centi | 1 m = 100 cm |
| Milli | 1 m = 1000 mm |
| Micro | 1 m = 1,000,000 m |
| Nano | n 1 m = 1,000,000,000 nm |
| Carbohydrates Monomer | glucose; Saccharide Test: Benedict’s test (+) Color: red/orange (-) Color: blue |
| Carb Dissacharides | lactose |
| Carb Polysaccharides | Starch; Polysaccharide Test: Lugol’s Iodine Test (+) Color: blue/black (-) Color: orange/brown |
| Lipids Monomer | glycerol, fatty acids Test: Emulsifier (+) Color: homogenous (-) Color: separated |
| Saturated Fats | Single bonds, ester bonds, Solid |
| Unsaturated Fats | Double bonds, Liquid |
| Proteins monomer | monomer: amino acids Structure: • central carbon • hydrogen atom H • radical group R • (2) functional groups 1. Amino group (NH2) 2. Carboxyl group (COOH) Test: Biuret test (+) Color: purple (-) Color: blue |
| Proteins polymer | Polymer: polypeptide Example: hemoglobin Test: Biuret test (+) Color: purple (-) Color: blue |
| Nucleic Acids monomer | Monomer: nucleotide (phosphate group, nitrogenous base, sugar) |
| Nucleic Acids polymer | Polymer: nucleic acid, no test |
| Carbohydrates | (sugars & starches) |
| Lipids | (fats, oils, & waxes) |
| Proteins | (enzymes & hemoglobin) |
| Nucleic Acid | DNA & RNA |
| Dehydration synthesis | larger macromolecules are built from smaller molecules by removing water; monomer to polymer |
| Hydrolysis | large macromolecules are broken down by adding water to form smaller molecules; polymer to monomer |
| 4 structures all cells (eukaryotic and prokaryotic) possess | • Plasma/cellular membrane • Cytosol/Cytoplasm • Genetic Material (DNA) • Ribosomes |
| major differences between eukaryotic and prokaryotic | • Their size (pro is bigger) • Pro DNA does not contain nucleus/moves freely in cell • Eukaryotic contains nucleus, stays in membrane • Cell structures • Pro represents monera kingdom/eukaryotic represents plants, animals, fungi, etc. |
| practice measurement conversions | |
| measurement equation | (starting/1) x (goal/starting) |
| Depth of Focus | the vertical distance thet remains in focus at any one time |
| Wet Mount | used for living specimens, place one drop of specimen on clean microscopic slide and cover with a cover slip |