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Bio Lab 4

QuestionAnswer
What four structures do ALL cells (eukaryotes AND prokaryotes) possess? Plasma/cellular membrane, cytosol/cytoplasm, genetic material (DNA), and Ribosomes
What are the major differences between eurkaryotes and prokaryotes? Their size (pro is bigger), Pro DNA does not contain nucleus/moves freely in cell, Eukaryotic contain nucleus/stays in membrane, cell structures, pro represents monera kingdom/eukaryotic represents plants, animals, fungi, etc.
Cell Wall maintains shape and structure, prevents bursting; in plants
Cell Membrane/Plasma controls what enters/exits the cell; in plants and animals
Nucleus contains DNA; maintains genes and controls activities of the cell; in plants and animals
Nucleolus produces ribosomes; in plants and animals
Ribosome produce proteins; in plants and animals
Rough Endoplasmic Reticulum processes and packages proteins, contain ribosomes; in plants and animals
Smooth Endoplasmic Reticulum No ribosomes; produces and processes lipids, in plants and animals
Golgi Apparatus Sorts and ships organic molecules, in plants and animals
Mitochondria convert organic materials to energy (ATP), in plants and animals
Lysosome contains digestive enzymes to break down organic molecules, in animals
Fat Vacuole storage of fat, in animals
Chloroplast site of photosynthesis, in plants
Central Vacuole Aid in cell division, In plants and animals
Cytoplasm Liquid component containing the organelles, in plants and animals
Gram Stain positive: dark purple/blue negative: red
Cell Morphology Coccus=round shape, Bacillus=rod shape, Spirillum=spiral shape
Practice Cell Structure Interactive Map
Magnification the amount an image is enlarged
Resolution=Resolving Power the extent to which image detail is preserved
Contrast the ability of an image to stand out against its background
Working Distance the vertical space between the front edge of the objective lens and the top surface of the coverslip or focused specimen
Field of view = Diameter multiply diameter (mm) by 1000 to get field of view (micrometers) ; The total circular or rectangular area of the specimen that you can see through the eyepiece or camera at one time.
Parfocal when you change lenses, image will always remain in focus
Parcentral when you change lenses, image will always stay in center of your field of view
Light Microscopes Characteristics: color images, living/non-living, 40x-1000x magnification, compound light and stereo dissecting
Light Microscope Types/uses Compound Light Microscope 2D, uses two lenses at a time, light goes up through specimen Stereo Dissecting Microscope 3D, light shines down on specimen to view outer surface detail
Electron Microscopes black/white images, non-living, 350000x magnification, creates electromagnetic field for magnification, Scanning Electron Microscope , 3D, outer images Transmission Electron Microscope 2D, internal structure
Scanning Power 4x, total magnification: 40x (red), shows the whole slide
Low Power 10x, total magnification: 100x (yellow), shows the object in greater detail
High Dry Power 40x, total magnification: 400x (blue), shows the object in even greater detail
Coarse Adjustment Knob Locates specimen, brings into approximate focus
Fine Adjustment Knob Brings specimen into final focus
Arm Supports upper parts of microscope and serves as a carrying handle
Base Bottom part of microscope, supports stage and arm
Body Connects nosepiece and eyepiece
Diaphragm Regulates the amount of light used to view the object
Eyepiece = Oculars series of lenses(top) through which an object is viewed , magnification is 10x
Light source=illuminator Lamp that directs a beam of light through the specimen
Revolving Nose Piece Revolving piece that holds objectives, lenses used to magnify specimen
Scanning Power Objective 4x, total magnification: 40x (red), shows the whole slide
Low Power Objective 10x, total magnification: 100x (yellow), shows the object in greater detail
High dry power Objective 40x, total magnification: 400x (blue), shows the object in even greater detail
Stage Holds and supports microscope slides
Slide Position Adjustment Knobs Moves slides left to right and front to back
Stage Clips Lever that opens to secure the slide on the stage
Label microscope image
Length The measurement of a line from one point to another Meter (m), Metric Ruler
Volume Amount of space an object occupies Liter (L), Erlenmeyer flask, beaker, graduated cylinder, pipet
Mass Amount of matter in an object Grams (g), Taring (setting scale to zero)
Temperature Degree of how hot or cold an object is Degrees C, Thermometer
Meniscus The curve of the liquid in the container (like a graduated cylinder).
Kilo 1 m = 1000 km
Deci 1 m = 10 cm
Centi 1 m = 100 cm
Milli 1 m = 1000 mm
Micro 1 m = 1,000,000 m
Nano n 1 m = 1,000,000,000 nm
Carbohydrates Monomer glucose; Saccharide Test: Benedict’s test (+) Color: red/orange (-) Color: blue
Carb Dissacharides lactose
Carb Polysaccharides Starch; Polysaccharide Test: Lugol’s Iodine Test (+) Color: blue/black (-) Color: orange/brown
Lipids Monomer glycerol, fatty acids Test: Emulsifier (+) Color: homogenous (-) Color: separated
Saturated Fats Single bonds, ester bonds, Solid
Unsaturated Fats Double bonds, Liquid
Proteins monomer monomer: amino acids Structure: • central carbon • hydrogen atom H • radical group R • (2) functional groups 1. Amino group (NH2) 2. Carboxyl group (COOH) Test: Biuret test (+) Color: purple (-) Color: blue
Proteins polymer Polymer: polypeptide Example: hemoglobin Test: Biuret test (+) Color: purple (-) Color: blue
Nucleic Acids monomer Monomer: nucleotide (phosphate group, nitrogenous base, sugar)
Nucleic Acids polymer Polymer: nucleic acid, no test
Carbohydrates (sugars & starches)
Lipids (fats, oils, & waxes)
Proteins (enzymes & hemoglobin)
Nucleic Acid DNA & RNA
Dehydration synthesis larger macromolecules are built from smaller molecules by removing water; monomer to polymer
Hydrolysis large macromolecules are broken down by adding water to form smaller molecules; polymer to monomer
4 structures all cells (eukaryotic and prokaryotic) possess • Plasma/cellular membrane • Cytosol/Cytoplasm • Genetic Material (DNA) • Ribosomes
major differences between eukaryotic and prokaryotic • Their size (pro is bigger) • Pro DNA does not contain nucleus/moves freely in cell • Eukaryotic contains nucleus, stays in membrane • Cell structures • Pro represents monera kingdom/eukaryotic represents plants, animals, fungi, etc.
practice measurement conversions
measurement equation (starting/1) x (goal/starting)
Depth of Focus the vertical distance thet remains in focus at any one time
Wet Mount used for living specimens, place one drop of specimen on clean microscopic slide and cover with a cover slip
Created by: bethanygumpel
 

 



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