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MI #1
| Question | Answer |
|---|---|
| An antigen-binding immunoglobulin, produced by B cells, that functions as the effector in an immune response. | Antibody |
| A foreign macromolecule that does not belong to the host organism and elicits an immune response. | Antigen |
| The collection, classification, storage, and analysis of biochemical and biological information using computers especially as applied in molecular genetics and genomics. | Bioinformatics |
| The amount of a specified substance in a unit amount of another substance. | Concentration |
| A protein serving as a catalyst; a chemical agent that changes the rate of reaction without being consumed by the reaction. | Enzyme |
| The complement of an organism's genes; an organism's genetic material. | Genome |
| Any measure whose purpose is to improve health or alter the course of disease. | Medical Intervention |
| A sudden rise in the incidence of a disease. | Outbreak |
| A specific causative agent of disease. | Pathogen |
| A molecule (a short strand of RNA or DNA) whose presence is required for formation of another molecule ( a longer chain of DNA) | Primer |
| A stepwise dilution of a substance in solution. | Serial dilution |
| A substance dissolved in another substance. | Solute |
| A homogeneous mixture of two or more substances, which may be solids, liquids, gases, or a combination of these. | Solution |
| A substance, usually a liquid, capable of dissolving another substance. | Solvent |
| The reactant on which an enzyme works. | Substrate |
| What is medical Intervention? | Any measure whose purpose is to improve health or alter the course of a disease. |
| What are the major medical intervention categories? | Prevention, medical devices, pharmaceuticals, diagnostics, treatment, and recovery, surgery, and genetics. |
| How does DNA sequencing work? (steps 1, 2, a, b, c) | 1. Isolate pathogen DNA with PCR, 2. Perform cycle sequencing -a. Denature DNA into template & complimentary sides with heat, b. add primers, polymerase, and free nucleotides, c. fragments end with fluorescent tags ( fragments are different lengths) |
| How does DNA sequencing work? (steps d, e) | d. Sequencer with tubes reads the fluorescent tabs with a laser beam, e. The computer displays peaks with As, Ts, C,s and Gs. |
| What does ELISA stand for? | Enzyme linked Immunosobrant Assay |
| What are the steps to an ELISA test? | Start with the antigen that binds to the well, then the primary antibody is added which binds to the antigen, then the secondary antibody is added ( with an enzyme attached ) and binds to the primary antibody, then a substrate is added; color changes. |
| What is neisseria meningitidis? | It is a bacteria that can cause severe invasive infections. Some symptoms include high fever, severe headache, and nausea. Higher risk groups include infants, teens, and college students in dorms. It is spread by close contact and respiratory droplets. |
| What is neisseria meningitidis? (PART 2) | It is diplococci shaped and gram negative. IV antibiotics can help fight against it. |
| What are you tell from the ELISA test? | Qualitative results are positive blue and negative clear, and quantitative results are determining how many antigens are present. |
| KNOW HOW TO DO A SERIAL DILUTION | (On one paper with tubes) :) |
| Who is Sue Smith and what did she do? | She is a college student that started an outbreak of meningitis on campus. She contracted it while visiting a friend. It spread through shared foods and drinks with friends, teammates, and classmates. |
| If a patient's original antigen concentration is very high, what would their serial dilution look like? | It would be a dark blue color. |
| What is the website used to test DNA to other disease DNA? | BLAST site |
| Who in Sue's group had meningitis? | Sue, Jill, Maria, and Marco. |
| What did Wanda and Alvin have? | EBV (Epstein-Barr virus), a type of herpes. |
| What did Anthony and Arnie have? | Influenza B |
| What did Maggie have? | Strep throat |
| What are treatment options for Sue and others with meningitis? | Since it is bacterial meningitis, she should go to the hospital and be given IV antibiotics. |
| What are some examples of medical interventions? | Vaccines, stethoscope, x-ray, MRI, and a cast. |
| How does the immune system work? | When B lymphocytes (B cells) spot a foreign substance, they create millions of antibodies into the bloodstream and the antibodies bind to the antigens and help destroy them. |
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