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Lab #1 Microscopy

TermDefinition
The course adjustment knob should only be used when the ________ objective is in place. scanning power
parfocal microscopes when you change lenses, image will always remain in focus
parcental microscopes when you change lenses, image will always stay in center of your field of view
magnification of ocular lens 10x
High Dry Power 40x, total magnification: 400x (blue), shows the object in even greater detail
Scanning Power 4x, total magnification: 40x (red), shows the whole slide
Low Dry Power 10x, total magnification: 100x (yellow), shows the object in greater detail
Why must specimens be thin for compound light microscopes? in order to let the light shine through them, light needs to pass through specimen
magnification the amount an image is enlarged
resolution/resolving power the extent to which image detail is preserved
contrast the ability of an image to stand out against its background
how to find field of view multiply diameter (mm) by 1000 to get field of view (micrometers)
Light Microscopes color images, living/non-living, 40x-1000x magnification, compound light and stereo dissecting
Electron Microscopes black/white images, non-living, 350000x magnification, creates electromagnetic field for magnification, scanning electron and transmission electron
Compound Light Microscope 2D, uses two lenses at a time, light goes up through specimen
Stereo Dissecting Microscope 3D, light shines down on specimen to view outer surface detail
Scanning Electron Microscope 3D, shows outer images
Transmission Electron Microscope 2D, shows internal structures, electrons pass through sample
Coarse Adjustment Knob Locates specimen, brings into approximate focus
Fine Adjustment Knob Brings specimen into final focus
Arm Supports upper parts of microscope and serves as a carrying handle
Base Bottom part of microscope, supports stage and arm
Body Connects nosepiece and eyepiece
Diaphragm Regulates the amount of light used to view the object
Eyepiece/Oculars series of lenses(top) through which an object is viewed
Light Source/Illuminator Lamp that directs a beam of light through the specimen
Revolving Nosepiece Revolving piece that holds objectives, lenses used to magnify specimen
Stage Holds and supports microscope slides
Slide Position Adjustment Knobs Moves slides left to right and front to back
Stage Clips Lever that opens to secure the slide on the stage
Depth of Focus the vertical distance thet remains in focus at any one time
Wet Mount used for living specimens, place one drop of specimen on clean microscopic slide and cover with a cover slip
Created by: user-1934014
 

 



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