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Microscope
Terms and meaning
| Question | Answer |
|---|---|
| What is the ocular lens? | |
| What is the ocular lens? | The eyepiece you look through on the microscope. |
| What is the magnification of the ocular lens? | 10X |
| What is the head of the microscope? | The upper portion of the microscope that supports the ocular lens. |
| What is the revolving nosepiece? | The part that holds the objective lenses and rotates to change objectives. |
| What is the function of the objective lenses? | They provide different levels of magnification. |
| What is the stage? | The platform where the microscope slide is placed. |
| What is the condenser? | The part below the stage that concentrates light onto the specimen. |
| What does the iris diaphragm lever do? | It controls the amount of light passing through the specimen. |
| What is the substage light? | The light source located below the stage. |
| What is the arm of the microscope? | The part that supports the upper portion of the microscope. |
| What does the fine adjustment knob do? | It makes small focusing adjustments. |
| What does the coarse adjustment knob do? | It makes large focusing adjustments. |
| What is the base of the microscope? | The bottom part that supports the microscope. |
| What does the light control do? | It adjusts the microscope light. |
| What are the 13 microscope parts shown on the diagram? | Ocular lens, head, arm, revolving nosepiece, objective lens, stage, condenser, iris diaphragm lever, substage light, fine adjustment knob, coarse adjustment knob, base, and light control. |
| What is the formula for total magnification? | Ocular lens magnification multiplied by objective lens magnification. |
| What color is the scanning objective? | Red |
| What is the magnification of the scanning objective? | 4X |
| What is the total magnification when using the scanning objective? | 40X |
| What color is the low-power objective? | Yellow |
| What is the magnification of the low-power objective? | 10X |
| What is the total magnification when using the low-power objective? | 100X |
| What color is the high-power objective? | Blue |
| What is the magnification of the high-power objective? | 40X |
| What is the total magnification when using the high-power objective? | 400X |
| What color is the oil-immersion objective? | White |
| What is the magnification of the oil-immersion objective? | 100X |
| What is the total magnification with the oil-immersion objective? | 1000X |
| Should the oil-immersion objective be used in this lab? | No. DO NOT USE the oil-immersion objective. |
| What is 10X ocular multiplied by a 4X objective? | 40X total magnification. |
| What is 10X ocular multiplied by a 10X objective? | 100X total magnification. |
| What is 10X ocular multiplied by a 40X objective? | 400X total magnification. |
| What is 10X ocular multiplied by a 100X objective? | 1000X total magnification. |
| Which objective should be used first to bring the letter e into focus? | The scanning-power objective. |
| How does a correctly oriented letter e appear when viewed through the microscope? | Upside down or inverted. |
| At what total magnification is the letter e viewed with the scanning objective? | 40X |
| What should you do after bringing the letter e into focus with the scanning objective? | Sketch what you see. |
| What should you do when repeating the letter e activity with the low-power objective? | Bring it into focus, sketch what you see, and measure the working distance. |
| At what total magnification is the letter e viewed with the low-power objective? | 100X |
| What happens to the field of view as magnification increases? | The field of view gets smaller. |
| Higher magnification causes what change in field of view? | A smaller field of view. |
| Lower magnification causes what change in field of view? | A larger field of view. |
| What should you do when repeating the letter e activity with the high-power objective? | Bring it into focus, sketch what you see, and measure the working distance. |
| At what total magnification is the letter e viewed with the high-power objective? | 400X |
| At 400X magnification, how much of the letter e can you see compared with lower magnification? | A smaller portion of the letter because the field of view is smaller. |
| What information is recorded in the microscopic summary chart? | Objective lens, sketch of the observed letter e, objective magnification, total magnification, working distance in mm, and diameter of field in mm. |
| Which objective lenses are listed on the microscopic summary chart? | Scanning, low-power, high-power, and oil-immersion. |
| What unit is used to record working distance? | Millimeters or mm. |
| What unit is used to record diameter of field? | Millimeters or mm. |
| What does parfocal mean? | The object stays in the same focal plane when changing objective lenses. |
| What happens to the specimen's focus on a parfocal microscope when changing objective lenses? | It stays mostly in focus because it remains in the same focal plane. |
| Which adjustment knob should be used when changing objective lenses? | The fine adjustment knob. |
| Why should you use the fine adjustment knob after changing objective lenses? | Only a small focusing adjustment should be needed on a parfocal microscope. |
| Which adjustment knob should NOT be used when changing objective lenses? | The coarse adjustment knob. |
| Why should you not use the coarse adjustment knob when changing objective lenses? | It can break the slide and/or damage the lens. |
| What can happen if you use the coarse adjustment knob at higher objective powers? | The slide and/or objective lens can be damaged. |
| What is an easy rule to remember when changing objective power? | Changing power means use fine focus. |
| Red objective means what? | Scanning objective, 4X objective magnification, and 40X total magnification. |
| Yellow objective means what? | Low-power objective, 10X objective magnification, and 100X total magnification. |
| Blue objective means what? | High-power objective, 40X objective magnification, and 400X total magnification. |
| White objective means what? | Oil-immersion objective, 100X objective magnification, and 1000X total magnification. Do not use it in this lab. |
| Scanning, low power, and high power correspond to what objective magnifications? | Scanning is 4X, low power is 10X, and high power is 40X. |
| Scanning, low power, and high power correspond to what total magnifications? | Scanning is 40X, low power is 100X, and high power is 400X. |
| What two major changes should you remember as microscope magnification increases? | The specimen appears larger and the field of view becomes smaller. |
| What happens to the amount of the specimen visible as magnification increases? | Less of the specimen is visible. |
| What are the two focusing knobs on the microscope? | Coarse adjustment knob and fine adjustment knob. |
| Which focusing knob makes large adjustments? | Coarse adjustment knob. |
| Which focusing knob makes small adjustments? | Fine adjustment knob. |
| Which microscope part holds the objective lenses? | Revolving nosepiece. |
| Which microscope part do you rotate to switch objective lenses? | Revolving nosepiece. |
| Which microscope part holds the slide? | Stage. |
| Which microscope part concentrates light on the specimen? | Condenser. |
| Which microscope part controls how much light passes through the specimen? | Iris diaphragm lever. |
| Which microscope part provides the light? | Substage light. |
| Which microscope part adjusts the light? | Light control. |
| Which microscope part supports the upper microscope? | Arm. |
| Which microscope part supports the entire microscope from the bottom? | Base. |
| Which microscope part do you look through? | Ocular lens. |
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