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Bio Unit 1: 1.1

TermDefinition
Latin root of 'Science' Derived from a Latin verb meaning 'to know'.
Scientific Inquiry A search for information and explanations of natural phenomena through an iterative process of observation and hypothesis testing.
Scientific Data Recorded observations upon which scientific inquiry is based.
Qualitative Data Descriptive, non-numerical recorded observations (e.g., field notes, behavior logs, photographs).
Quantitative Data Numerical measurements organized into tables, charts, or graphs and analyzed with statistics.
Inductive Reasoning Logic that derives general principles from a large set of specific observations (Specific -> General).
Deductive Reasoning Logic that flows from general premises to specific predicted results using 'If... then' logic (General -> Specific).
Hypothesis An explanation based on observations and assumptions that leads to testable predictions.
Experiment A scientific procedure carried out under controlled conditions to test a hypothesis.
Proof vs. Support for Hypotheses A hypothesis can never be proven true beyond all doubt; testing only fails to disprove it, building confidence in its validity.
Limits of Scientific Testing Science can only test natural, observable phenomena; supernatural or religious explanations cannot be tested or falsified.
Falsifiability The principle that a scientific hypothesis must be capable of being proven false by an observation or experiment.
Observation: Mouse Camouflage Case Study Beach mice have light coat coloration on white sand, while inland mice of the same species have dark coats on dark soil.
Hypothesis: Mouse Camouflage Case Study Coat color patterns evolved as adaptations that camouflage mice from visual predators in their respective habitats.
Independent Variable (IV) The factor that is intentionally manipulated or changed by the experimenter.
Dependent Variable (DV) The factor being measured to see if it is affected by the independent variable.
Independent Variable: Mouse Experiment The coat color of the plastic mouse models (light vs. dark).
Dependent Variable: Mouse Experiment The proportion of predator attack marks recorded on the plastic mouse models.
Control Group: Mouse Experiment Mouse models matching their native habitat background color (light mice on beach; dark mice inland).
Experimental Group: Mouse Experiment Mouse models with non-matching coloration for their habitat (dark mice on beach; light mice inland).
Results: Mouse Camouflage Case Study Non-camouflaged models suffered significantly higher attack rates in both beach and inland environments.
Controlled Experiment An experiment designed to compare an experimental group with a control group to cancel out the effects of unwanted variables.
Scientific Theory A broad explanation supported by a massive body of evidence that generates many new, testable hypotheses.
Hypothesis vs. Theory A hypothesis is a narrow explanation for a specific observation; a theory is a comprehensive, widely supported framework.
Iterative Process of Science Science rarely follows a strict linear pathway; hypotheses are continually revised and retested as new data emerge.
Basis of Metric System A decimal measurement system based on factors of 10.
Base Unit for Length Meter (m)
Base Unit for Liquid Volume Liter (L)
Base Unit for Mass/Weight Gram (g)
Kilo- (k) 1,000 units (10^3)
Hecto- (h) 100 units (10^2)
Deca- (da / dk) 10 units (10^1)
Deci- (d) 0.1 units (10^-1)
Centi- (c) 0.01 units (10^-2)
Milli- (m) 0.001 units (10^-3)
Micro- (µ) 0.000001 units (10^-6)
Nano- (n) 10^-9 units
Pico- (p) 10^-12 units
Femto- (f) 10^-15 units
Metric Mnemonic Device King Henry Died Unusually Drinking Chocolate Milk (Kilo, Hecto, Deca, Unit, Deci, Centi, Milli).
Converting to a SMALLER unit MULTIPLY by 10 for each step right (move decimal point to the right).
Converting to a LARGER unit DIVIDE by 10 for each step left (move decimal point to the left).
1 Millimeter benchmark About the thickness of a dime.
1 Centimeter benchmark About the width of a paperclip or fingernail.
1 Meter benchmark About the width of a doorway.
1 Kilometer benchmark About 10 football fields or 6 city blocks (~0.62 miles; 1.6 km ≈ 1 mile).
1 Gram benchmark About the weight of a small paperclip.
1 Kilogram benchmark About 6 apples or 2.2 pounds.
1 Milligram benchmark About the weight of a single grain of salt.
1 Liter benchmark Half of a 2-liter soda bottle (slightly more than a quart).
1 Milliliter benchmark Equal to about 2 drops of liquid.
1 Microliter (µL) 1/1000th of a mL (smaller than a single tiny drop).
Volume Formula (Rectangular Prism) Volume = Length x Width x Height (measured in cubic units like cm³).
Relationship: 1 Liter to cm³ 1 Liter = 1,000 cubic centimeters (cm³ or cc).
1 Cubic Centimeter (cm³) equivalent 1 cm³ = 1 mL
Meniscus The curved upper surface of a liquid in a tube or container.
How to read a Meniscus correctly View dead-on at eye level and read the volume at the BOTTOM of the curve.
Parallax Error Inaccurate reading caused by looking at the meniscus from an angle (above or below eye level).
Beaker / Erlenmeyer Flask Accuracy Low accuracy; used for general holding/mixing (5–5000 mL).
Graduated Cylinder Used for rapid liquid volume measurement; semi-accurate (~1% tolerance).
Volumetric Flask Very high accuracy for preparing solutions to one specific, precise volume.
TC (To Contain) marking Indicates the container holds the specified volume when filled to the calibration mark.
Proper technique for filling Pipettes Keep the tapered end beneath the liquid surface at all times; use a bulb or pipet pump (NEVER mouth pipet).
Serological Pipette Calculation Rule Zero is at the top; subtract desired volume from total capacity to find target mark (e.g., to deliver 6.5 mL with a 10 mL pipet, fill to 3.5 mL).
Pasteur / Transfer Pipette Low accuracy glass or plastic pipettes used for uncalibrated/rough transfer (1–5 mL).
Micropipette (Pipettor) High-precision instrument used to measure very small volumes in microliters (µL).
P10 Range 0.5 µL to 10 µL
P20 Range 2 µL to 20 µL
P200 Range 20 µL to 200 µL
P1000 Range 200 µL to 1000 µL (1 mL)
Micropipette First Stop Fills or measures the exact calibrated volume (evacuates the preset air volume).
Micropipette Second Stop Blows out remaining liquid to ensure complete delivery (+50% extra travel).
Micropipette Common Errors Bad/damaged tip, wrong tip size, air bubbles, liquid not at room temperature, forcing adjustment past limits.
Multi-channel Pipette Pipette that holds 4–16 tips at once to increase efficiency (commonly used in ELISA assays).
Created by: Cocoalover
 

 



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