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microbio quiz #3
| Question | Answer |
|---|---|
| catalase tests the | presence of catalase |
| medium and reagents for catalase test | glass slide 3% hydrogen peroxide |
| positive result for catalase test | bubbles |
| nitrate test tests the | conversion of nitrate to nitrite or to ammonia or nitrogen gas |
| medium and reagents for nitrate test | nitrate broth nitrate reagents A &B zinc dust (if needed) |
| results for nitrate test | red (before zinc) - positive red (after zinc) - negative |
| mannitol salt agar tests for | halophiles and fermentation |
| mannitol salt agar medium | MSA |
| bacitracin and novobiocin medium | SBA plates |
| zone of inhibition | sensitive |
| coagulase test medium | coagulase plasma from rabbits |
| Hemolysis testing medium | SBA |
| alpha vs beta vs gamma hemolysis | alpha - partial (green) beta - complete (clear) gamma - none (no zone) |
| Voges proskauer test (VP) tests for | production of a neutral product (acetoin) from fermentation |
| voges proskauer test medium and reagents | MR-VP reagent VP reagents A & B, creatine |
| voges proskauer positive test is | red |
| growth in 6.5% NaCl tests for what, medium, results | tests for halophiles, salt broth, turbidity = positive |
| bile esculin tests for the | hydrolysis of esculin to esculetin |
| bile esculin medium and results | bile esculin agar slant black = positive |
| starch hydrolysis tests for | the presence of amylase in bacteria, which hydrolyzes starch |
| medium and reagent for starch hydrolysis test | starch agar plate iodine |
| positive result for starch hydrolysis | clear zone (starch has been hydrolyzed) |
| optochin sensitivity medium and results | SBA zone of inhibition of 14 mm or more = sensitive zone less than 14 mm = resistant |
| motility test is done in what medium | sulfur indole motility agar (SIM) |
| mannose fermentation medium | cystein tryptic agar w/ mannose (CTA-M) |
| mannose fermentation positive results | yellow color |
| casein agar tests the | hydrolysis of casein |
| medium for casein. | skim milk agar |
| positive result for casein | clear area |
| methyl red (MR) tests for the | production of acidic end product from fermentation |
| medium and reagents for MR | MR-VP broth methyl red |
| ONPG tests for | beta galactose hydrolyzing ONPG to ortho-nitrophenol |
| ONPG medium | ONPG broth |
| positive ONPG | yellow |
Created by:
kingsleya27