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Ochem 337 Final

TermDefinition
What governs the distribution of the solutes between the aqueous phase and the organic phase? They are governed by their relative stabilities
How can one ascertain whether a layer is the organic phase or the aqueous phase? 1. Add a drop of mystery layer to 2 mL of water 2. If the drop dissolves its from the aqueous phase 3. If drop doesn't dissolve its from the organic phase
Why must extraction mixtures be vented? Most organic solvents are volatile (evaporate easily) which generates pressure in the seperatory funnel
What three advantages are associated with the thin-layer chromatographic technique? 1. Small sample size 2. Easy to perform and rapid 3. Good resolution
What two limitations are associated with gas-liquid chromatography? 1. Limited to a small sample sizes 2. Limited to reasonably volatile samples
What are the seven basic parts of a gas-liquid chromatography? 1. Source of high-pressure pure carrier gas (hydrogen or helium) 2. Gas flow controller 3. Heated injector port 4. Column 5. Column oven 6. Detector 7. Recording device or data station
What three pieces of information are provided in the data table of a gas chromatography? 1. Peak retention times 2. Peak areas 3. Percent compositions ( area %)
What must be available in order for GC analysis to permit compound identification? Authentic samples of the various components in the mixture being analyzed
What are the six items that must be determined in order to establish the sequence of a peptide (or protein)? 1. Cleave all disulfide bridges (DB) + alkylate resulting thiols 2. Determine constituent amino acids (kind and numbers) 3. Identify N-terminal AA 4. Identify C-terminal AA 5. Fragmentation through partial hydrolysis 6. Identify locations of any DB's
An enzyme that catalyzes the hydrolysis of a peptide bond at the end of the peptide chain is called? Exopeptidase
What conditions are employed to "completely" hydrolyze a peptide? 1. 6M HCl 2. 100 degrees C 3. 24 hours
Created by: user-2043933
 

 



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