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microbio midterm
| Question | Answer |
|---|---|
| viruses are composed of | nucleic acid surrounded by a protective protein coat. |
| prokaryotes | no membrane bound nucleus and organelles |
| Observation of viruses requires | the use of an electron microscope. |
| swabbing allows | easy detection of microbes |
| simple microscope | only has one lens |
| manitol salt agar is ___% salt | 7.5 |
| example of a halophile | staph |
| what color does the plate turn if the bacteria ferments the mannitol on a MSA plate? | yellow |
| staph. epidermidis on a MSA --> staph. aureus on a MSA --> | no color change yellow |
| PEA | phenyl ethyl alcohol agar |
| strep viridans | group of normal, non-pathogenic strep, G+ |
| strep pyogens | pathogenic, causes strep throat beta |
| alpha vs beta vs gamma hemolysis | alpha - complete hemolysis (clear area) beta - partial (green) gamma - no hemolysis |
| SLS streptolysin vx. SLO streptolysin O | SLS - oxygen stable SLO - oxygen labile |
| what was the mueller hinton agar plates used for? | antibiotic resistance |
| after a spore stain, the bacteria is | red |
| what staining procedure is best for viewing capsules | negative |
| if G+ is over decolorized, it will be | pink |
| uricult tubes contain the media | CLED and EMB |
| streptococcus pyogenes has ____ hemolysis | beta |
| heat fixing does what? | adheres the bacteria to the slide |
| negative stain uses an ___________ dye | acidic |
| simple staining uses the dye | methylene blue |
| spore staining requires the use of | a steam bath |
| gram staining tells you what | what kind of cell wall a bacteria has |
| gram + vs gram - | gram + has a thick layer of peptidoglycan (purple) gram - only has a thin layer of peptidoglycan and stains pink |
| steps to a gram stain | put bacteria on slide (add drop of water if needed) heat fix primary stain (1 min) then rinse mordant (1 min) - makes stain stick better decolorizer (10 secs) counterstain - safranin (1 min) |
| acid fast bacteria contains | mycolic acid |
| macconkeys agar is ________________ and ______________ | selective and differential |
| SBA is | TSA + 5% sheep blood |
| BHI | Brain heart infusion contains extracts of cow brains and hearts |
| when bacteria grows in a broth, | the liquid gets cloudy and turbid |
| deeps detect ______________ | motility |
| What type of microorganism from lab 1 causes anthrax | bacillus |
| what is the legal limit for bacteria in municipal drinking water | 1/100 mL |
| urine bacteria can be | 10,000-100,000 anything over indicates infection |
Created by:
kingsleya27