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Microbiology lab Exa
| Question | Answer |
|---|---|
| What part of the microscope do you look through? | Ocular lens (eyepiece) |
| What is the usual magnification of the ocular lens? | 10x |
| Which microscope part holds the objective lenses? | Nosepiece |
| What microscope part holds the slide? | Stage |
| What focus knob should be used with the 100x lens? | Fine adjustment only |
| What can happen if coarse focus is used on 100x? | Damage to slide/lens |
| Why must oil be cleaned after use? | Prevent lens damage and blurry images |
| What should be used to clean microscope lenses? | Lens paper and lens cleaner only |
| Which objective should be down during storage? | 10x objective |
| What should happen to light intensity before storage? | Turn low/off |
| What does parfocal mean? | Image stays mostly focused when changing objectives |
| What does parcentric mean? | Object stays centered when changing objectives |
| Which bacteria are rod-shaped? | Bacillus |
| Which bacteria appear in grape-like clusters? | Staphylococci |
| Which organism is bell-shaped? | Vorticella |
| Which organism appears as green spirals? | Spirogyra |
| Which protozoan is slipper-shaped? | Paramecium |
| Which objective should you begin focusing with? | 10x objective |
| Which focus knob is used at 40x? | Fine adjustment |
| What must be adjusted as magnification increases? | : Light intensity and iris diaphragm |
| What type of microscope was used in this lab? | Compound light microscope |
| Why are microscopes called compound microscopes? | They use two lenses (ocular + objective) |
| Colony morphology | The visible characteristics of bacterial growth on agar |
| Configuration | The overall shape of a bacterial colony |
| Margin | The edge or border of a bacterial colony |
| Elevation | The side-view height or profile of a colony |
| Texture | The appearance or feel of a colony |
| Color and sheen | The pigment and reflective appearance of a colony |
| Circular | A perfectly round colony shape |
| Irregular | An uneven or asymmetrical colony shape |
| Broth liquid media | A liquid medium used to grow bacteria |
| Clear broth | Little or no bacterial growth in broth |
| Turbid broth | Cloudy broth caused by bacterial growth |
| Precipitation | Growth settled at the bottom of broth |
| Flocculation | Clumps floating in broth |
| Pellicle | A film of bacterial growth on top of broth |
| Colony | A visible mass of bacteria descended from one cell |
| Positive pressure room | A room used to protect immunocompromised patients |
| Negative pressure room | A room used to isolate airborne infections like tuberculosis |
| Living surfaces | Usually contain more microbes but fewer types |
| Nonliving surfaces | Usually contain a greater variety of microbes |
| Tuberculosis isolation | Requires a negative pressure room |
| Mucoid colonies | Commonly appear slimy due to capsule production |
| The five major colony morphology characteristics | Configuration margin elevation texture color |
| Convex elevation | A dome-shaped colony elevation |
Created by:
Kerry12