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microbio quiz #1
labs 1 & 2
| Question | Answer |
|---|---|
| prokaryotes | no membrane bound nucleus or organelles (bacteria) |
| eukaryotes | have a nucleus + organelles (fungi, protozoans, parasites) |
| viruses are composed of | nucleic acid surrounded by a protective protein coat or phospholipid bilayer |
| how is detection of microbes achieved | swabbing |
| simple vs compound microscopes | 1 lens vs multiple lens |
| who invented the first microscope? | anton van leeuwenhoek |
| amoeba | single celled eukaryotic organism; freshwater environments; forms pseudopods |
| yeast | unicellular, eukaryotic fungus; grows naturally on fruit surfaces; reproduces asexually; ferments sugars to produce ethanol and CO2 |
| mold | multicellular fungi organized into a network of filaments called mycelium |
| hyphae | individual filaments that absorb nutrients |
| penicillium | reproduces asexually; og source of penicillin |
| flagellates | eukaryotic parasites that move by flagella |
| most rods belong to the family | enterobacteriaceae |
| bacillus | gram + bacteria that are long, rod shaped, and form chains |
| staphylococcus forms | clusters |
| streptococcus forms | chains |
| gram - coccus causes | gonorrhea and meningitis |
| mesophiles | grow at room temp (20-25C) to body temp (37C) |
| psychrophiles | grow at low temps (4*C) |
| thermophiles | grow at high temps (50*) |
| aerobic | need oxygen to grow |
| anaerobic | grow without oxygen |
| facultative | can grow in anaerobic and aerobic conditions |
| capnophilic | grow in high concentrations of CO2 |
| broth | liquid medium |
| agar | solid medium; polysaccharide from seaweed |
| TSA/ TSB | trypticase soy agar/ broth; nutrients from soybeans grow a wide variety of bacteria |
| SBA | sheep's blood agar; addition of 5% sheep's blood to TSA |
| BHI | brain heart infusion; supports the growth of most bacteria with cow brains and hearts |
| what tool is used to move bacteria? | inoculating loop |
| what tool is used to inoculate a deep/ stab? | inoculating needle |
| what technique is used to separate bacteria? | streaking plates |