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CC - PRELIM

L1 - LAB

QuestionAnswer
Measurement of the intensity of light at selected wavelengths Spectrophotometry
Is the distance between two peaks as the light travels in a wavelike manner Wavelength
Wavelength; UV LESS than 300 nm
Wavelength; VL 300-700 nm
Wavelength; Infrared MORE THAN 700nm
Beer’s Law / Beer-Lambert Law ; PRINCIPLE The concentration of a substance is directly proportional to the amount of light absorbed or inversely proportional to the logarithm of the transmitted light
Beer’s Law / Beer-Lambert Law ; Equation 𝐴 = π‘Žπ‘π‘ 𝐴 = 2 βˆ’ π‘™π‘œπ‘”%οΏ½
Is used to measure the light transmitted by a solution to determine the concentration of the light-absorbing substance in the solution Spectrophotometer
Components of a Spectrophotometer 1. Light source 2. Monochromator 3. Sample cell/ cuvette 4. Photodetectors
Isolates radiant energy (light) of a desired wavelength Monochromator
A small vessel used to hold a liquid sample to be analyzed in the light path of a spectrometer o It may beround, square, or rectangular Sample cell/ cuvette
A device that converts the transmitted radiant energy into an equivalent amount of electrical energy Photodetectors
Created by: twisa_heart
 

 



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