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genetics final
genetics
| Term | Definition |
|---|---|
| restriction enzymes | produced by bacteria as a defense mechanism against bacteriophage infection. Restrict infections by degrading the viral DNA. Binds to DNA at a specific nucleotide sequence and cuts both strands of DNA within the sequence by cleaving phosphodiester bonds. |
| Fragment Link | Joins DNA fragments together using DNA Ligase to glue the backbone together. |
| RFLP (Restriction Fragment Length Polymorphism) | Identifies variation in DNA sequences by cutting DNA into different fragment sizes. |
| ASO (Allele specific oligonucleotide) | specific DNA probes used to detect point mutations, which are SNPs |
| SNPs | They're variations in DNA sequences from person to person. They're short, single stranded and hybridize complementary alleles/sequences. |
| WGS | Whole genome sequencing. Part of personal genomics. Entire genome. |
| GWAs | organizes whole populations. can be graphed. |
| Xist | encodes long noncoding RNA that coats the X chromosome. Results in changes in chromatin structure that SILENCE transcription. Turns off the inactive X chromosome. Recruits PCR2. |
| Tsix gene | transcribed on the active X chromosome. Antisense to Xist. Represses the encoding of Xist on the X chomosome, letting one X chomosome be active. |
| Jpx gene | encodes lncRNA that stimulates the transcription of Xist on the inactive X chromosome. Basiclly activates Xist on the inactive X chromosome. |
| Xite gene | sustains Tsix. |
| PCR2 | proteins produce epigenetic marks that repress transcription, Recruited by Xist. |