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Recombinant DNA

QuestionAnswer
Name one way of altering a wild microbe 1. mutagenesis 2. Recombinant DNA technology
Define mutagenesis Deliberately exposing microbes to UV light to induce an advantageous mutation
Explain why mutagenesis of wild microbes occurs To try to create an improved strain of microbe through advantageous mutations
Define recombinant DNA technology Plant/animal genes inserted into microbes to produce plant/animal proteins
Describe the role of endonuclease Same endonuclease is used to cut open plasmid and cut gene from chromsome
Explain why the same restriction endonuclease is used To create complementary sticky ends between gene and plasmid
Explain the role of ligase To seal gene into plasmids
Name the process when plasmids are inserted into bacterial cells Transformation
Explain why yeast vectors are preferable over bacterial plasmids Only yeast vectors can fold the protein correctly making protein active
Define a vector DNA molecule used to carry foreign genetic material into a cell.
Name the two types of vectors 1. plasmids 2. artificial chromosomes
Which vector is preferable when inserting a larger gene Artificial chromosomes
Explain the role of ORI self replication of the plasmid
Explain the role of regulatory sequences Control gene expresssion
Give an example of a selectable marker 1. Antibiotic resistance 2. fluorescence
Explain the process by which only transformed bacteria can be detected using an antibiotic resistance selectable marker Expose all bacteria to antibiotics & only those that have been selectively transformed have resistance and survive
If a selectable marker has been interrupted will it still confer antibiotic resistance to the bacteria No - gene not functional and protein cant be made
Created by: brightminds
 

 



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