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Lab practical final
| Question | Answer |
|---|---|
| lactose fermentation test | uses emb , phynl red or MacConkey agar. positive result generates acid and gas |
| Eosin Methylene Blue (EMB) agar | inhibits growth of gram positive bacteria. tests for lactose fermentation, positive result pink or purple, E coli is green |
| Durham Lactose Broth | tests for lactose fermentation. glass vile inside a larger tube. positive result has cloudiness and a gas bubble |
| phenol red | used in urea test. tests for acid production. naturally red-orange. turns yellow in acid. turns pink in alkaline. |
| MacConkey (MAC) agar | tests for lactose fermentation. inhibits growth of gram positves. default yellow, turns pink when positve result |
| Methyl green | visual indicator for DNase Test Agar. A clear halo or colorless zone surrounding the bacterial growth is a positive result |
| Toluidine Blue O | visual indicator for DNase Test Agar. for positive result a bright pink halo forms around the colonies |
| indole test | test for ability to make tryptophanase. done via Tryptone Broth or SIM media. pink ring forms on surface for positive result. |
| Tryptic soy/Tryptone Broth | liquid medium used primarily to perform the Indole Test. needs Kovac's Reagent. |
| SIM (Sulfide-Indole-Motility) medium/media/agar | three-in-one semi-solid agar that tests for Sulfide production, Indole production, and Motility |
| Methyl Red (MR) test | determine if a bacterium performs mixed-acid fermentation. red if positive, yellow if negative, orange if mixed or inconclusive |
| Optochin | Antibiotic used to differentiate between s pnumoniae from other bacterium. Positive result as zone of inhibition |
| Blood agar | Used to observe hemolysis. Alpha = partial: greenish or brownish. Beta = complete: clear zone around cells. Gamma = none |
| Bacitracin | Antibiotic used to differentiate S pyogenes from other streptococcus |
| Catalase test | Tests for aerobic bacteria. Positive is vigorous bubbling when reagent of hydrogen peroxide is added. |
| Mannitol Salt Agar (MSA) | Isolates Staphylococcus, who like salty environments. S. aureus is yellow, ferments the mannitol making acid. S. Epi makes it pink |
| DNase test | Tests for a dna breaker enzyme. Uses HCl, methyl green, or toluidine blue. Halo forms around colonies in positive result. |
| Triple Sugar Iron (TSI) slant agar | Tests for ability to ferment certain sugars and production of H2S gas. Red is neutral, yellow is acidic, pink is basic, Black is H2S. |
| Carb fermintation test | Used to tell if can ferment a specific sugar. Uses phenol red and inverted glass tube. Yellow+for acid, bubble in tube for gas+ |
| Urease test | urease hydrolyzes urea into ammonia and carbon dioxide, pink is positive, |
| Voges-Proskauer (VP) test | if a bacterium ferments glucose via the butanediol pathway, making acid. positive is pink or red, negative is yellow or brown |
| Citrate Utilization Test | if live on only sodiumcitrate for carbon and ammoniumsalt for nitrogen. uses SimmonsCitrateAgar. +Intense Blue or growth |
| Simmons Citrate Agar | used for the citrate test |
| Reagents for the gram stain | Crystal violet, grams iodine, acetone-isopropanol deodorizer, and safranin |
| Reagents for acid fast | Carbolfuchsin, acid isopropanol decolorizer, methylene blue |
| skim milk agar | has casein, turns media white, bacteria exoenzyme breaks casein, making white ring around bacteria |
| spirit blue agar | has lipids and spirit blue and pH indicator. breakdown of lipid makes acid and clear line in the dark blue. |
| starch agar | tests for amylase enzyme. clear area around bacteria is positive. lugol's iodine is blue black, gram's is light brown |
| IMVIC | indole, methyl red, voges-proskauter, citrate |
| bile solubility test | tests if cell wall dissolves in bile salts, clearing around cells is positive result |
| durham tube | small tube inside bigger tube |
| nitrate reduction test | two reagents and zinc used. red means nitrate reduced to nitrite, clear means the nitrite was also reduced to ammonia or nitrogen |
| Oxidase test | positive result, quick dark purple/blue color change, identifies bacteria that produce the enzyme cytochrome c oxidase |
| amylase test (or starch hydrolysis test) | inoculating starch agar, incubating it, and adding iodine; a clear zone around growth indicates a positive test, blue black is neg |
Created by:
DinoRex