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lab practical 3

QuestionAnswer
results for Serratia gram -, orange colonies, DNase+
results for Pseudomonas Gram -, blue-green colonies, oxidase +, carb fermentation -
Results for E. coli Gram -, neutral color, ferment lactose, indole +, MR+, VP-, Citrate-
Results for Enterobacter Gram -, neutral color, ferment lactose, indole -, MR-, VP+, Citrate+
Results for psudomonas Gram -, neutral color, does not ferment lactose, no production of H2S, oxidase +, carb fermentation-
Results for proteus Gram - , neutral color, does not ferment lactose, yes production of H2S, produce urease, indole +
Results for salmonella Gram - , neutral color, does not ferment lactose, yes production of H2S
Results for Staphylococcus epidermis Gram + , have catalase bubbles, growth on MSA, no ferment manatol, coagulase - , dnase -
Results for micrococcus luteus Gram+ , have catalase bubbles, no growth on MSA
Results for Staphylococcus aureus Gram + , have catalase bubbles, growth on MSA, yes ferment manatol, coagulase + , dnase +
Results for streptococcus pyogenes Gram +, no catalase bubbles, beta hemalysis, sensitive to bacitracin,
Results for Enterococcus faecium Gram +, no catalase bubbles, beta hemalysis, not sensitive bacitracin
Results for Streptococcus pnumoniae Gram +, no catalase bubbles, alpha or gamma hemalysis, sensitive to optochin, bile solubility +
Results for Enterococcus faecalis Gram +, no catalase bubbles, alpha or gamma hemalysis, not sensitive to optochin
lactose fermentation test uses emb , phynl red or MacConkey agar. positive result generates acid and gas
Eosin Methylene Blue (EMB) agar inhibits growth of gram positive bacteria. tests for lactose fermentation, positive result pink or purple, E coli is green
Durham Lactose Broth tests for lactose fermentation. glass vile inside a larger tube. positive result has cloudiness and a gas bubble
phenol red used in urea test. tests for acid production. naturally red-orange. turns yellow in acid. turns pink in alkaline.
MacConkey (MAC) agar tests for lactose fermentation. inhibits growth of gram positves. default yellow, turns pink when positve result
DNase test (or DNA hydrolysis test) tests for production of DNase, which breaks down dna for food or disguise, uses methyl green or Toluidine Blue O
Methyl green visual indicator for DNase Test Agar. A clear halo or colorless zone surrounding the bacterial growth is a positive result
Toluidine Blue O visual indicator for DNase Test Agar. for positive result a bright pink halo forms around the colonies
indole test test for ability to make tryptophanase. done via Tryptone Broth or SIM media. pink ring forms on surface for positive result.
Tryptone Broth liquid medium used primarily to perform the Indole Test. needs Kovac's Reagent.
SIM (Sulfide-Indole-Motility) medium/media/agar three-in-one semi-solid agar that tests for Sulfide production, Indole production, and Motility
Methyl Red (MR) test determine if a bacterium performs mixed-acid fermentation. red if positive, yellow if negative, orange if mixed or inconclusive
Optochin Antibiotic used to differentiate between s pnumoniae from other bacterium. Positive result as zone of inhibition
Blood agar Used to observe hemolysis. Alpha = partial: greenish or brownish. Beta = complete: clear zone around cells. Gamma = none
Bacitracin Antibiotic used to differentiate S pyogenes from other streptococcus
Catalase test Tests for aerobic bacteria. Positive is vigorous bubbling when reagent of hydrogen peroxide is added.
Mannitol Salt Agar (MSA) Isolates Staphylococcus, who like salty environments. S. aureus is yellow, ferments the mannitol making acid. S. Epi makes it pink
Coagulase test Determines whether bacterium produces coagulase. Positive result is clumping
DNase test Tests for a dna breaker enzyme. Uses HCl, methyl green, or toluidine blue. Halo forms around colonies in positive result.
Triple Sugar Iron (TSI) slant agar Tests for ability to ferment certain sugars and production of H2S gas. Red is neutral, yellow is acidic, pink is basic, Black is H2S.
Carb fermintation test Used to tell if can ferment a specific sugar. Uses phenol red and inverted glass tube. Yellow+for acid, bubble in tube for gas+
Urease test urease hydrolyzes urea into ammonia and carbon dioxide, pink is positive,
Voges-Proskauer (VP) test if a bacterium ferments glucose via the butanediol pathway, making acid. positive is pink or red, negative is yellow or brown
Citrate Utilization Test if live on only sodiumcitrate for carbon and ammoniumsalt for nitrogen. uses SimmonsCitrateAgar. +Intense Blue or growth
Simmons Citrate Agar used for the citrate test
Created by: DinoRex
 

 



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