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Bio Lab
| Question | Answer |
|---|---|
| A positive test for Urea will cause the pH to increase making the test tube ___ color. | Pink |
| SIM media is testing for | sulfur reduction, indole production, and motility |
| Cloudiness in the tube represents | motility |
| redness when Kovac's agent is added in the SIM media represents | indole production |
| A black SIM tube represents | sulfur reduction |
| On a Triple Sugar tube, what does a red slant yellow butt represent? Red and Red? | fermentation of glucose occurred (but not sucrose or lactose) ; fermentation of all |
| Simmons Citrate Agar, if it turns blue... | The bacteria uses citrate. |
| Select the chemicals which are required for the Voges-Proskauer (VP) test. | 1-napthol potassium hydroxide |
| methyl red test, the test tube appears red. | positive for mixed acid fermentation |
| Following the Voges-Proskauer test, the test tube appears copper. | negative for acetoin production |
| Is MacConkey agar both selective and differential? Explain | Only gram negative can grow on it, differential due to lactose presence |
| For which types of microbes is MacConkey agar selective? | Gram negative enteric bacteria |
| Identify the true statement regarding growth of bacteria on MacConkey agar: | If capable of lactulose fermentation, colonies will appear pink |
| Identify which of the following is NOT assessed with SIM media: | antibiotic effectiveness |
| What is urease? | Urease is an enzyme produced by some bacteria; it breaks down urea to form carbon dioxide and ammonia. |
| MSA plates grow gram positive bacteria. If yellow they are able to perfrom | mannitol fermentation |
| Partial hemolysis; blood agar plate appears olive green. | Alpha Hemolysis |
| Full hemolysis; a clearing is observed where red blood cells were completely destroyed. | Beta Hemolysis |
| Non-hemolytic pattern; no breakdown of red blood cells is observed. | gamma hemolysis |
| incidence of infection | the number of new cases |
| prevalence of infection | cumulative number of individuals |
| pandemic | when epidemic reaches global scale |
| Bacteria resistant to multiple antibiotics are known as, list an example | superbugs, mrsa |