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mole. genetics ch.18

RNA splicing and processing

QuestionAnswer
what is splicing? the removal of introns from RNA, resulting in mature RNA
what is a spliceosome? a large splicing apparatus that takes the form of an array of proteins and ribonucleoproteins that functions as a large complex
what is autonomous splicing? introns that catalyze their own removal from a precursor RNA transcript without the need for a spliceosome
how are eukaryotic mRNAs processed?
what makes up the 5' cap? a guaning residue attached thorugh a 5'-5' triphosphate linkage. the g reside is in the reverse orientation from all other nucleotides
how is the poly(A) tail made? the mature 3' ends of RNAP II-transcribed mRNA are generated by cleavage followed by polyadenylation, which is necessary for the maturation of mRNA from nuclear RNA
what is the function of the 5' cap? protects the mRNA from degradation
what is the function of the 3' tail? protects the mRNA from degradation by 3'-5' exonucleases
what is a splice site? sequences immediately surrounding the exon-intron boundaries
what is a splice site sequence? specific sequences in precursor mRNA where the spliceosome binds to remove introns and join exons during RNA splicing
what is a branch point? an important sequence that is located anywhere from 18 to 40 nucleotides upstream from tjhe 3' end of an intron
what is the sequence of a branch point? YNYYRAY, where Y indicates a pyrimidine, N denotes any nucleotide, R denotes any purine, and A denotes adenine
what is a snRNP? proteins that catalyze splicing
what are auto-catalytic introns? introns that excise themselves from RNA by an autocatalytic splicing event
Created by: angievelasco
 

 



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