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mole. genetics ch.17
eukaryotic transcription
| Question | Answer |
|---|---|
| what is a transcription factor? | any protein that is required for the initiation of transcription, but is not a part of RNA polymerase |
| what is the biggest difference between transcribing genes in prokaryotes versus eukaryotes? | in eukaryotes, transcription takes place on a densely proteinized chromatin template |
| what is a promoter? | DNA sequence primarily upstream of a gene that is crucial for the initiation of transcription |
| what is the difference between RNA polymerase I, II, and III? | RNAP I transcribes rRNAs, RNAP II transcribes mRNA and other snRNAs, and RNAP III transcribes tRNA, rRNA, and other snRNAs |
| what is RNA polymerase I used for and where is it found? | found exclusively in the nucleolus of eukaryotes and is used to transcribe rDNA into rRNA |
| what is RNA polymerase II used for and where is it found? | it is found in the nucleus of eukaryotes and it transcribes DNA into mRNA as well as other snRNAs |
| what is RNA polymerase III used for and where is it found? | it is found in the nucleoplasm of eukaryotes and it transcribes short noncoding RNA including tRNA, rRNA, and other snRNAs |
| where are the promoters for RNA polymerase I, II, and III found? | for RNAP I and II it is found upstream of the start point, where RNAP III is found downstream of the start point |
| what is an enhancer? | sequence that increases the utilization of eukaryotes promoters and can function in either orientation and in any location (upstream or downstream) relative to the promoters |
| what is a silencer? | a short sequence of DNA that can inactivate the expression of a gene in its vicinity |
| where are enhancers found within the genome? | they can be found upstream, inside, or beyond the end of a gene |
| how do enhancers work? | they interact with protein bound at the promoter elements, often through intermediates called co-activators |
| what is a housekeeping gene? | essential, constitutive genes that maintain basic cellular functions |
| what is TBP? | (TATA binding protein) is a component of the positioning factor that is required for each type of RNA polymerase to bind to its promoter. binds directly to TATA box when present, but is required even when not present |
| what polymerases use TBP? | all 3 polymerases use TBP |
| what are the different stages of chromatin? | closed chromatin, permissive chromatin, and open chromatin |
| what is a poised gene? | a potentially active gene in open chromatin |